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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown; lipolytic enzyme involved in cellular metabolism.
ProductProbable lipase/esterase LipG
CommentsRv0646c, (MTCY20H10.27c), len: 301 aa. Probable lipG, lipase/esterase, equivalent to NP_302278.1|NC_002677 probable hydrolase from Mycobacterium leprae (304 aa). Also highly similar to various hydrolases, especially lipases e.g. AA61351.1|X88895 carboxyl esterase from Acinetobacter calcoaceticus (312 aa), FASTA scores: opt: 867, E(): 0, (50.2% identity in 279 aa overlap); etc. Also similar to transferases e.g. P77026 macrolide 2'-phosphotransferase II from Escherichia coli (279 aa), FASTA scores: E(): 1.3e-14, (32.5% identity in 286 aa overlap). Similar to M. tuberculosis non-heme bromoperoxidases and epoxide hydrolases.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003).
MutantDisruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS740234741139-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0646c|lipG
VDIRSGTAVSGDVKLYYEDMGDLDHPPVLLIMGLGAQMLLWRTDFCARLVAKGLRVIRYDNRDVGLSTKTERHRPGQPLATRLVRSWLGLPSQAAYTLEDMAADAAALLDHLDVKHAHVVGASMGGMIAQIFAARFAQRTKTLAVIFSSNNHRFLPPPAPRALLALLTGPPPDSPRDVIVDNAVRVSKIIGSPAYPIPEDQVRAEAAESYDRNFHPWGIAQQFSAILGSGSLLRYDRRIVAPTVVIHGRADKLMRPFGGRAVARAINGARLVLIDGMGHDLPRQLWDRVIGELTRNFSEAG