Gene Rv0782 (ptrBa)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Cleaves peptide bonds on the C-terminal side of lysyl and argininyl residues [catalytic activity: hydrolysis of ARG-|-XAA and LYS-|-XAA bonds in oligopeptides, even when P1' residue is proline]. |
| Product | Probable protease II PtrBb [second part] (oligopeptidase B) |
| Comments | Rv0782, (MTCY369.26), len: 552 aa. Probable ptrBb, second part of protease II, equivalent to C-terminus of NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. Belongs to peptidase family S9A; also known as the prolyl oligopeptidase family. Note that previously known as ptrBa. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Intermediary metabolism and respiration |
| Transcriptomics | DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 874732 | 876390 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0782|ptrBb
VTNDIPCGSRIYAPENSTRTRSPGSERESPGQLTTTVYYTTVDAAWRPDTVWRYRLGSGESSERVYHEADDRFWLAVGRTRSNAYLLIAAGSSITSEVRYAHAADPTAQFSVVLPRRDGVEYSVEHAVIAGQDRFLILHNDGAVNFTLVEAPVEDPARQRTLIAHRDDVRLDAVDALAGHLVVSYRREALPRVQLWPIGPDGNYGEPEEISFDSELMSAGLGPNPNWDSPKLRVGAGSFVTPVRIYDIDLVTGERTLLKEQPVLGGYRREDYVERRDWAYGDDGTRIPVSIVHRADIEFPAPALIYGYGAYEICEDPRFSIARLSLLDRGMVFVVAHVRGGGEMGRLWYENGKLLDKKNTFTDFIAVARHLVDTGLTSQQQLVALGGSAGGLLMGAVANMAPDLFAGILAQVPFVDPLTTILDPSLPLTVTEWDEWGNPLNDSDVYAYVKSYSPYENVTAQKYPAILAMTSLNDTRVYYVEPAKWVAALRHAKTDGNSVLLKTQMHAGHGGISGRYERWKETAFQYGWLLATADSDRYGGGQGNDLDGAAPA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
