Gene Rv0981
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Regulator part of a two component regulatory system (MPRAB system) |
| Product | Mycobacterial persistence regulator MRPA (two component response transcriptional regulatory protein) |
| Comments | Rv0981, (MTV044.09), len: 228 aa. MprA, mycobacterial persistence regulator, a two-component response regulator whose expression is required for entrance into and maintenance of persistent infection (see citation below), equivalent to NP_301250.1|NC_002677 putative two-component response regulator from Mycobacterium leprae (228 aa); and highly similar to others from Mycobacterium leprae. Also highly similar to others e.g. AAG36759.1|AF119221_1|AF119221 response regulator from Corynebacterium glutamicum (232 aa); CAB88489.1|AL353816 putative two-component system response regulator from Streptomyces coelicolor (248 aa); BJY09666_1 two-component response regulator (ragA, ragB and rpoH3) from B.japonicum (226 aa), FASTA score: (43.8% identity in 224 aa overlap); BSAJ2571_44 two-component response regulator from Bacillus subtilis (228 aa), FASTA score: (46.4% identity in 224 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. Rv1033c (257 aa); Rv0903c (236 aa), FASTA score: (50.7 identity in 225 aa overlap); etc. Contains PS00217 Sugar transport proteins signature 2. Start changed since first submission (-2 aa). MprAB is involved in the regulation of genes in response to environmental stress (See He et al., 2006). |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). DNA microarrays and qRT-PCR indicate regulation by MprA under physiological conditions and environmental stress (SDS and Triton X-100) (See He et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv Rv0981 mutant has tissue-specific and stage-specific attenuation in mice (See Zahrt and Deretic 2001). M. tuberculosis H37Rv mprA|Rv0981-mprB|Rv0982 mutant has increased survival in vitro in the presence of 0.05% SDS; growth rate in human monocytes is higher than wild-type (See Pang et al., 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1096822 | 1097508 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0981|mprA
VRILVVDDDRAVRESLRRSLSFNGYSVELAHDGVEALDMIASDRPDALVLDVMMPRLDGLEVCRQLRGTGDDLPILVLTARDSVSERVAGLDAGADDYLPKPFALEELLARMRALLRRTKPEDAAESMAMRFSDLTLDPVTREVNRGQRRISLTRTEFALLEMLIANPRRVLTRSRILEEVWGFDFPTSGNALEVYVGYLRRKTEADGEPRLIHTVRGVGYVLRETPP
Bibliography
- Zahrt TC et al. [2001]. Mycobacterium tuberculosis signal transduction system required for persistent infections. Mutant Function Regulation
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Zahrt TC et al. [2003]. Functional analysis of the Mycobacterium tuberculosis MprAB two-component signal transduction system. Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- He H et al. [2006]. MprAB is a stress-responsive two-component system that directly regulates expression of sigma factors SigB and SigE in Mycobacterium tuberculosis. Function Regulation Transcriptome
- Pang X et al. [2007]. Evidence for complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
