Gene Rv1299
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA |
| Product | Probable peptide chain release factor 1 PrfA (RF-1) |
| Comments | Rv1299, (MTCY373.19), len: 357 aa. Probable prfA, peptide chain release factor 1 (rf-1), highly similar to many e.g. RF1_MYCLE|P45833 peptide chain release factor 1 (rf-1) from Mycobacterium leprae (357 aa), FASTA scores: opt: 2047, E(): 0, (89.3% identity in 356 aa overlap); also similar to Mycobacterium tuberculosis Rv3105c, prfB peptide chain release factor 2. Contains PS00745 Prokaryotic-type class I peptide chain release factors signature. Belongs to the prokaryotic and mitochondrial release factors family. |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Transcriptomics | DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1455495 | 1456568 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1299|prfA
MTQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDDLETARELVASDESFAAEVAALEARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATLAIASKADTPDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNTTDSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIRTVDRSERIRTYNFPENRITDHRIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
