Gene Rv1406
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Modify the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTU-GTP. [catalytic activity: 10-formyltetrahydrofolate + L-methionyl-tRNA + H(2)O = tetrahydrofolate + N-formylmethionyl-tRNA] |
| Product | Probable methionyl-tRNA formyltransferase Fmt |
| Comments | Rv1406, (MTCY21B4.23), len: 312 aa. Probable fmt, methionyl-tRNA formyltransferase, similar to many e.g. FMT_ECOLI|P23882 Escherichia coli (314 aa), FASTA scores: opt: 616, E(): 6.7e-31, (39.3% identity in 303 aa overlap). Belongs to the FMT family. |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1582166 | 1583104 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1406|fmt
VRLVFAGTPEPALASLRRLIESPSHDVIAVLTRPDAASGRRGKPQPSPVAREAAERGIPVLRPSRPNSAEFVAELSDLAPECCAVVAYGALLGGPLLAVPPHGWVNLHFSLLPAWRGAAPVQAAIAAGDTITGATTFQIEPSLDSGPIYGVVTEVIQPTDTAGDLLKRLAVSGAALLSTTLDGIADQRLTPRPQPADGVSVAPKITVANARVRWDLPAAVVERRIRAVTPNPGAWTLIGDLRVKLGPVHLDAAHRPSKPLPPGGIHVERTSVWIGTGSEPVRLGQIQPPGKKLMNAADWARGARLDLAARAT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
