Gene Rv1683
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Supposed involvement in lipid biosynthesis and degradation |
| Product | Possible bifunctional enzyme; long-chain acyl-CoA synthase and lipase. |
| Comments | Rv1683, (MTCI125.05), len: 999 aa. Possible bifunctional long-chain acyl-CoA synthase and lipase. Equivalent to Z95117|MLCB1351_21 possible long-chain acyl-CoA synthase from Mycobacterium leprae (1002 aa) (85.6% identity in 1002 aa overlap). Weakly similar to FATP_MOUSE|Q60714 long-chain fatty acid transport protein (646 aa), fasta scores: opt: 331, E(): 5e-08, (24.8% identity in 630 aa overlap). Also similar to O35488|AF033031 Mouse very-long-chain acyl-CoA synthetase (620 aa), fasta scores: opt: 435, E(): 2.2e-12, (24.8% identity in 545 aa overlap). Weakly similar to Mycobacterium tuberculosis protein MTCI364.18 (27.4% identity in 583 aa overlap). Contains PS00120 Lipases, serine active site. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1907594 | 1910593 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1683|Rv1683
VVDLNFSMVTRPIERLVATAQNGLEVLRLGGLETGSVPSPSQIVESVPMYKLRRYFPPDNRPGQPPVGPPVLMVHPMMMSADMWDVTREDGAVGILHASGLDPWVIDFGSPDEVEGGMRRNLADHIVALSEAVDTVKDATGHDVHFVGYSQGGMFCYQAAAYRRSKDIASVVAFGSPVDTLAALPMGIPANMGAAVADFMADHVFNRLDIPSWMARMGFQMMDPLKTAKARVDFVRQLHDREALLPREQQRRFLESEGWIAWSGPAISELLKQFIAHNRMMTGGFAISGQMVTLTDITCPILAFVGEVDDIGQPASVRGIRRAAPNSEVYECLIRAGHFGLVVGSRAAQQSWPTVADWVRWISGDGTKPENIHLMADQPAEHTDSGVAFSSRVAHGIGEVSEAALALARGAADAVVAANRSVRTLAVETVRTLPRLARLGQLNDHTRISLGRIIDEQAHDAPKGEFLLFDGRVHTYEAVNRRINNVVRGLIAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVVMRPDTDLSASVRLGRVTEILTDPTNLDAARQLPGQVLVLGGGESRDLDLPADALEQGQVIDMEKIDPDAVELPAWYRPNPGLARDLAFIAFSSADGDLVAKQITNYRWAVSAFGTASTAALGRRDTVYCLTPLHHESALLVSLGGAVVGGTRIALSRGLRPDRFVAEVRQYGVTVVSYTWAMLRDVVDDPAFVLHGNHPVRLFIGSGMPTGLWERVVEAFAPAHVVEFFATTDGQAVLANVAGAKIGSKGRPLPGAGRVELGAYDAEHDLILENDRGFVQVAGVNQVGVLLAQSRGPIDPTASVKRGVFAPADTWISTDYLFWRDDDGDYWLAGGRGSVVRTARGMVYTEPVTNALGLITGVDLAVTYGVLVRGRHVAVSAVTLLPGATITAADLTEAVASMPVGLGPDIVHVVPQLTLSGTYRPTVSALRANGIPKAGRQAWYFNSGGNEYRRLTPAVRTELTGQHRRGNA
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Low KL et al. [2010]. Lipid droplet-associated proteins are involved in the biosynthesis and hydrolysis of triacylglycerol in Mycobacterium bovis bacillus Calmette-Guerin. Function Product
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
