Gene Rv1783
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | ESX conserved component EccC5. ESX-5 type VII secretion system protein. |
| Comments | Rv1783, (MTV049.05-MTV049.06), len: 1391 aa. eccC5, esx conserved component, ESX-5 type VII secretion system protein, probable membrane protein. FtsK/SpoIIIE family protein. Similar to Rv3894c. Member of family of Mycobacterium tuberculosis hypothetical proteins including Rv3447c, Rv0284, Rv3870, Rv1783, Rv3871, Rv3894c, all linked to ESAT-6 family genes. Equivalent to Mycobacterium leprae hypothetical protein Q9Z512|MLCB596.28|AL035472 (1345 aa). Previously annotated as two separate genes eccCa5|Rv1783 and eccCb5|Rv1784, now fused due to A:T correction at position 2020563 resulting in *463L. Contains two times PS00017 ATP/GTP-binding site motif A (P-loop). Former Rv1784 - Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011) (Rv1783/Rv1784). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2019257 | 2023432 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1783|eccC5
MKRGFARPTPEKPPVIKPENIVLSTPLSIPPPEGKPWWLIVVGVVVVGLLGGMVAMVFASGSHVFGGIGSIFPLFMMVGIMMMMFRGMGGGQQQMSRPKLDAMRAQFMLMLDMLRETAQESADSMDANYRWFHPAPNTLAAAVGSPRMWERKPDGKDLNFGVVRVGVGMTRPEVTWGEPQNMPTDIELEPVTGKALQEFGRYQSVVYNLPKMVSLLVEPWYALVGEREQVLGLMRAIICQLAFSHGPDHVQMIVVSSDLDQWDWVKWLPHFGDSRRHDAAGNARMVYTSVREFAAEQAELFAGRGSFTPRHASSSAQTPTPHTVIIADVDDPQWEYVISAEGVDGVTFFDLTGSSMWTDIPERKLQFDKTGVIEALPRDRDTWMVIDDKAWFFALTDQVSIAEAEEFAQKLAQWRLAEAYEEIGQRVAHIGARDILSYYGIDDPGNIDFDSLWASRTDTMGRSRLRAPFGNRSDNGELLFLDMKSLDEGGDGPHGVMSGTTGSGKSTLVRTVIESLMLSHPPEELQFVLADLKGGSAVKPFAGVPHVSRIITDLEEDQALMERFLDALWGEIARRKAICDSAGVDDAKEYNSVRARMRARGQDMAPLPMLVVVIDEFYEWFRIMPTAVDVLDSIGRQGRAYWIHLMMASQTIESRAEKLMENMGYRLVLKARTAGAAQAAGVPNAVNLPAQAGLGYFRKSLEDIIRFQAEFLWRDYFQPGVSIDGEEAPALVHSIDYIRPQLFTNSFTPLEVSVGGPDIEPVVAQPNGEVLESDDIEGGEDEDEEGVRTPKVGTVIIDQLRKIKFEPYRLWQPPLTQPVAIDDLVNRFLGRPWHKEYGSACNLVFPIGIIDRPYKHDQPPWTVDTSGPGANVLILGAGGSGKTTALQTLICSAALTHTPQQVQFYCLAYSSTALTTVSRIPHVGEVAGPTDPYGVRRTVAELLALVRERKRSFLECGIASMEMFRRRKFGGEAGPVPDDGFGDVYLVIDNYRALAEENEVLIEQVNVIINQGPSFGVHVVVTADRESELRPPVRSGFGSRIELRLAAVEDAKLVRSRFAKDVPVKPGRGMVAVNYVRLDSDPQAGLHTLVARPALGSTPDNVFECDSVVAAVSRLTSAQAPPVRRLPARFGVEQVRELASRDTRQGVGAGGIAWAISELDLAPVYLNFAENSHLMVTGRRECGRTTTLATIMSEIGRLYAPGASSAPPPAPGRPSAQVWLVDPRRQLLTALGSDYVERFAYNLDGVVAMMGELAAALAGREPPPGLSAEELLSRSWWSGPEIFLIVDDIQQLPPGFDSPLHKAVPFVNRAADVGLHVIVTRTFGGWSSAGSDPMLRALHQANAPLLVMDADPDEGFIRGKMKGGPLPRGRGLLMAEDTGVFVQVAATEVRR
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Niemann S, Koser CU, Gagneux S, Plinke C, Homolka S, Bignell H, Carter RJ, Cheetham RK, Cox A, Gormley NA, Kokko-Gonzales P, Murray LJ, Rigatti R, Smith VP, Arends FP, Cox HS, Smith G and Archer JA [2009]. Genomic diversity among drug sensitive and multidrug resistant isolates of Mycobacterium tuberculosis with identical DNA fingerprints. Sequence
- Ioerger TR et al. [2010]. Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories. Sequence
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
