Gene Rv1809
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PPE family protein PPE33 |
| Comments | Rv1809, (MTV049.31), len: 468 aa. PPE33, Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, most similar to RV1802AL022021|MTV049.23 (463 aa), FASTA scores: opt: 1238, E(): 0, (51.2% identity in 471 aa overlap). Alternative nucleotide at position 2051746 (T->C; A155A) has been observed. |
| Functional category | Pe/ppe |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h, 24h, and 96h of starvation (see citation below). DNA microarrays show higher level of expression in M. tuberculosis H37Rv during Mg2+ starvation (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2051282 | 2052688 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1809|PPE33
MDFGLQPPEITSGEMYLGPGAGPMLAAAVAWDGLAAELQSMAASYASIVEGMASESWLGPSSAGMAAAAAPYVTWMSGTSAQAKAAADQARAAVVAYETAFAAVVPPPQIAANRSQLISLVATNIFGQNTAAIAATEAEYGEMWAQDTMAMFGYASSSATASRLTPFTAPPQTTNPSGLAGQAAATGQATALASGTNAVTTALSSAAAQFPFDIIPTLLQGLATLSTQYTQLMGQLINAIFGPTGATTYQNVFVTAANVTKFSTWANDAMSAPNLGMTEFKVFWQPPPAPEIPKSSLGAGLGLRSGLSAGLAHAASAGLGQANLVGDLSVPPSWASATPAVRLVANTLPATSLAAAPATQIPANLLGQMALGSMTGGALGAAAPAIYTGSGARARANGGTPSAEPVKLEAVIAQLQKQPDAVRHWNVDKADLDGLLDRLSKQPGIHAVHVSNGDKPKVALPDTQLGSH
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Golby P, Nunez J, Cockle PJ, Ewer K, Logan K, Hogarth P, Vordermeier HM, Hinds J, Hewinson RG and Gordon SV [2008]. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Regulon
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
