Gene Rv1983
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PE-PGRS family protein PE_PGRS35 |
| Comments | Rv1983, (MTCY39.36c), len: 558 aa. PE_PGRS35, Member of the Mycobacterium tuberculosis PE family, PGRS subfamily of gly-rich proteins (see Brennan & Delogu 2002). Similar to other PE proteins e.g. Rv0977, etc. Contains PS00141 Eukaryotic and viral aspartyl proteases active site. |
| Functional category | Pe/ppe |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Transcriptomics | mRNA identified by RT-PCR (see Banu et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2226244 | 2227920 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1983|PE_PGRS35
VSFLVVVPEFLTSAAADVENIGSTLRAANAAAAASTTALAAAGADEVSAAVAALFARFGQEYQAVSAQASAFHQQFVQTLNSASGSYAAAEATIASQLQTAQHDLLGAVNAPTETLLGRPLIGDGAPGTATSPNGGAGGLLYGNGGNGYSATASGVGGGAGGSAGLIGNGGAGGAGGPNAPGGAGGNGGWLLGNGGIGGPGGASSIPGMSGGAGGTGGAAGLLGWGANGGAGGLGDGVGVDRGTGGAGGRGGLLYGGYGVSGPGGDGRTVPLEIIHVTEPTVHANVNGGPTSTILVDTGSAGLVVSPEDVGGILGVLHMGLPTGLSISGYSGGLYYIFATYTTTVDFGNGIVTAPTAVNVVLLSIPTSPFAISTYFSALLADPTTTPFEAYFGAVGVDGVLGVGPNAVGPGPSIPTMALPGDLNQGVLIDAPAGELVFGPNPLPAPNVEVVGSPITTLYVKIDGGTPIPVPSIIDSGGVTGTIPSYVIGSGTLPANTNIEVYTSPGGDRLYAFNTNDYRPTVISSGLMNTGFLPFRFQPVYIDYSPSGIGTTVFDHPA
Bibliography
- Brennan MJ et al. [2002]. The PE multigene family: a 'molecular mantra' for mycobacteria. Review
- Banu S et al. [2002]. Are the PE-PGRS proteins of Mycobacterium tuberculosis variable surface antigens? Product Transcriptome Localization Clinical
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
