Gene Rv2187
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) |
| Comments | Rv2187, (MTV021.20), len: 600 aa. fadD15, long-chain-fatty-acid-CoA ligase, similar to several e.g. P44446|LCFH_HAEIN putative long-chain-fatty-acid--CoA ligase from Haemophilus influenzae (607 aa), FASTA scores: (607 aa) opt: 992, E(): 0, (31.5% identity in 578 aa overlap); etc. Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by proteomics at the Statens Serum Institute (Denmark) (see Rosenkrands et al., 2000). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2448160 | 2449962 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2187|fadD15
VREISVPAPFTVGEHDNVAAMVFEHERDDPDYVIYQRLIDGVWTDVTCAEAANQIRAAALGLISLGVQAGDRVVIFSATRYEWAILDFAILAVGAVTVPTYETSSAEQVRWVLQDSEAVVLFAETDSHATMVAELSGSVPALREVLQIAGSGPNALDRLTEAGASVDPAELTARLAALRSTDPATLIYTSGTTGRPKGCQLTQSNLVHEIKGARAYHPTLLRKGERLLVFLPLAHVLARAISMAAFHSKVTVGFTSDIKNLLPMLAVFKPTVVVSVPRVFEKVYNTAEQNAANAGKGRIFAIAAQTAVDWSEACDRGGPGLLLRAKHAVFDRLVYRKLRAALGGNCRAAVSGGAPLGARLGHFYRGAGLTIYEGYGLSGTSGGVAISQFNDLKIGTVGKPVPGNSLRIADDGELLVRGGVVFSGYWRNEQATTEAFTDGWFKTGDLGAVDEDGFLTITGRKKEIIVTAGGKNVAPAVLEDQLRAHPLISQAVVVGDAKPFIGALITIDPEAFEGWKQRNSKTAGASVGDLATDPDLIAEIDAAVKQANLAVSHAESIRKFRILPVDFTEDTGELTPTMKVKRKVVAEKFASDIEAIYNKE
Bibliography
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Arora P et al. [2009]. Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
