Gene Rv2212
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in cAMP synthesis [catalytic activity: ATP = 3',5'-cyclic AMP + diphosphate] |
| Product | Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase) |
| Comments | Rv2212, (MTCY190.23), len: 378 aa. Adenylyl cyclase (See Abdel Motaal et al., 2006). Some similarity to e.g. SW:CYAA_STRCO P40135 adenylate cyclase (29.2% identity in 291 aa overlap); ttg at 24614 in MTCY190 has a better rbs. Contains possible helix-turn-helix motif at aa 64- 85, (+2.72 SD). Also similar to Rv1264 and Rv1647 |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in the culture filtrate and whole cell lysates of M. tuberculosis H37Rv but not the membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2477190 | 2478326 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2212|Rv2212
VYDSLDFDALEAAGIANPRERAGLLTYLDELGFTVEEMVQAERRGRLFGLAGDVLLWSGPPIYTLATAADELGLSADDVARAWSLLGLTVAGPDVPTLSQADVDALATWVALKALVGEDGAFGLLRVLGTAMARLAEAESTMIRAGSPNIQMTHTHDELATARAYRAAAEFVPRIGALIDTVHRHHLASARTYFEGVIGDTSASVTCGIGFADLSSFTALTQALTPAQLQDLLTEFDAAVTDVVHADGGRLVKFIGDAVMWVSSSPERLVRAAVDLVDHPGARAAELQVRAGLAYGTVLALNGDYFGNPVNLAARLVAAAAPGQILAAAQLRDMLPDWPALAHGPLTLKGFDAPVMAFELHDNPRARDADTPSPAASD
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Abdel Motaal A et al. [2006]. Fatty acid regulation of adenylyl cyclase Rv2212 from Mycobacterium tuberculosis H37Rv. Function Product
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
