Gene Rv2236c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in cobalamin biosynthesis, in the conversion of cobyric acid to cobinamide. |
| Product | Probable cobalamin biosynthesis transmembrane protein CobD |
| Comments | Rv2236c, (MTCY427.17c), len: 313 aa. Probable cobD, cobalamin biosynthesis transmembrane protein, similar to S52223 Rhodobacter capsulatus 945 protein BluD (39.0% identity in 287 aa overlap) involved in cobinamide synthesis, and to COBD_PSEDE Pseudomonas dentrificans cobD protein (37.5% identity in 269 aa overlap), also CBIB_SALTY Salmonella typhimurum cbiB protein (35.5% identity in 304 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2508434 | 2509375 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2236c|cobD
VFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRSWPVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWAASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHPSPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP
Bibliography
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
