Gene Rv2333c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transport of drug across the membrane (export) |
| Product | Integral membrane drug efflux protein Stp |
| Comments | Rv2333c, (MTCY3G12.01), len: 537 aa. stp, integral membrane drug efflux protein (See Ramon-Garcia et al., 2007), member of major facilitator superfamily (MFS), highly similar to many e.g. Q9RL22|C5G9.04c putative transmembrane efflux protein from Streptomyces coelicolor (489 aa), FASTA scores: opt: 1031, E(): 4e-55, (37.4% identity in 412 aa overlap); Q9L0L9|SCD82.12 putative transmembrane efflux protein from Streptomyces coelicolor (490 aa), FASTA scores: opt: 883, E(): 3.8e-46, (36.35% identity in 407 aa overlap); Q9ZBW5|SC4B5.03c putative integral membrane efflux protein from Streptomyces coelicolor (504 aa), FASTA scores: opt: 899, E(): 4.1e-47, (37.4% identity in 415 aa overlap); P39886|TCMA_STRGA tetracenomycin C resistance and export protein from Streptomyces glaucescens (538 aa), FASTA scores: opt: 839, E(): 1.9e-43, (32.3% identity in 489 aa overlap); etc. Also highly similar to Rv2459|O53186|MTV008.15 probable conserved integral membrane transport protein from Mycobacterium tuberculosis strain H37Rv (508 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.05% identity in 504 aa overlap); and AAK46834|MT2534 drug transporter from Mycobacterium tuberculosis strain CDC1551 (523 aa), FASTA scores: opt: 1385, E(): 1.5e-76, (44.4% identity in 504 aa overlap). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2606708 | 2608321 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2333c|stp
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLDGRRRWYLIGVSLFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALFVWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVALLVATATLAVVFLAGWRWFPRDVHTAGSDLSERLPTAMTVECAVSHMPGATWCRLWPA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Ramón-García S et al. [2007]. Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG. Function Product
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
