Gene Rv2343c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | DNA primase is the polymerase that synthesizes small RNA primers for the okazaki fragments on both template strands replication FORKS during chromosomal DNA synthesis. |
| Product | Probable DNA primase DnaG |
| Comments | Rv2343c, (MTCY98.12c), len: 639 aa. Probable dnaG, DNA primase, equivalent to O52200|PRIM_MYCSM|DNAG DNA primase from Mycobacterium smegmatis (636 aa), FASTA scores: opt: 3504, E(): 5.5e-202, (81.55% identity in 639 aa overlap); and Q9CCG2|DNAG|ML0833 DNA primase from Mycobacterium leprae (642 aa), FASTA scores: opt: 3443, E(): 2.5e-198, (80.4% identity in 642 aa overlap). Also highly similar to many DNA primases e.g. Q9S1N4|PRIM_STRCO|DNAG|SC7A8.07c from Streptomyces coelicolor (641 aa), FASTA scores: opt: 1899, E(): 5.1e-106, (47.9% identity in 643 aa overlap); P74893|PRIM_SYNP7|DNAG from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (616 aa), FASTA scores: opt: 860, E(): 6.6e-44, (35.3% identity in 513 aa overlap); P05096|PRIM_BACSU from Bacillus subtilis (603 aa) FASTA scores: opt: 800, E(): 2.5e-40, (33.7% identity in 430 aa overlap); etc. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2620533 | 2622452 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2343c|dnaG
MSGRISDRDIAAIREGARIEDVVGDYVQLRRAGADSLKGLCPFHNEKSPSFHVRPNHGHFHCFGCGEGGDVYAFIQKIEHVSFVEAVELLADRIGHTISYTGAATSVQRDRGSRSRLLAANAAAAAFYAQALQSDEAAPARQYLTERSFDAAAARKFGCGFAPSGWDSLTKHLQRKGFEFEELEAAGLSRQGRHGPMDRFHRRLLWPIRTSAGEVVGFGARRLFDDDAMEAKYVNTPETLLYKKSSVMFGIDLAKRDIAKGHQAVVVEGYTDVMAMHLAGVTTAVASCGTAFGGEHLAMLRRLMMDDSFFRGELIYVFDGDEAGRAAALKAFDGEQKLAGQSFVAVAPDGMDPCDLRLKCGDAALRDLVARRTPLFEFAIRAAIAEMDLDSAEGRVAALRRCVPMVGQIKDPTLRDEYARQLAGWVGWADVAQVIGRVRGEAKRTKHPRLGRLGSTTIARAAQRPTAGPPTELAVRPDPRDPTLWPQREALKSALQYPALAGPVFDALTVEGFTHPEYAAVRAAIDTAGGTSAGLSGAQWLDMVRQQTTSTVTSALISELGVEAIQVDDDKLPRYIAGVLARLQEVWLGRQIAEVKSKLQRMSPIEQGDEYHALFGDLVAMEAYRRSLLEQASGDDLTA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
