Gene Rv2384
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the biogenesis of the hydroxyphenyloxazoline-containing siderophore mycobactins (initiation step of mycobactin chain growth). Activates the mycobactin ArCP in two half-reactions: activates salicylic acid as acyladenylate (adenylation step) + transfers activated salicylate to the MBTA ArCP as a thioester (arylation step). |
| Product | Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase |
| Comments | Rv2384, (MTCY22H8.01, MTCY253.37c), len: 565 aa. mbtA, bifunctional enzyme, including salicyl-AMP ligase (Sal-AMP ligase) and salicyl-S-ArCP synthetase (see Quadri et al., 1998; De Voss et al., 1999), highly similar to other ligases e.g. Q9F638|MXCE from Stigmatella aurantiaca 2,3-DHBA-AMP ligase (protein involved in the biosynthesis of 2,3-dihydroxybenzoic acid, contains the AMP binding signature) (543 aa), FASTA scores: opt: 1683, E(): 2.8e-90, (48.25% identity in 545 aa overlap) (see Silakowski et al., 2000); P40871|DHBE_BACSU|ENTE 2,3-dihydroxybenzoate-AMP ligase from Bacillus subtilis (539 aa), FASTA scores: opt: 1569, E(): 1.2e-83, (44.9% identity in 532 aa overlap); O07899|VIBE_VIBCHVC0772 vibriobactin-specific 2,3-dihydroxybenzoate-AMP ligase from Vibrio cholerae (543 aa), FASTA scores: opt: 1457, E(): 3.7e-77, (44.6% identity in 545 aa overlap); etc. Also similar to P95819|SNBA pristinamycin I synthetase I from Streptomyces pristinaespiralis (582 aa), FASTA scores: opt: 1532, E(): 1.7e-81, (46.35% identity in 548 aa overlap); and Q9RFM9|PCHD salicyl-AMP ligase from Pseudomonas aeruginosa (547 aa), FASTA scores: opt: 1415, E(): 1e-74, (45.95% identity in 533 aa overlap). Contains PS00455 Putative AMP-binding domain signature. Belongs to the ATP-dependent AMP-binding enzyme family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). |
| Transcriptomics | DNA microarrays indicate repression by iron and IdeR|Rv2711 in M. tuberculosis H37Rv (See Rodriguez et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2675936 | 2677633 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2384|mbtA
MPPKAADGRRPSPDGGLGGFVPFPADRAASYRAAGYWSGRTLDTVLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAATGLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGPFVSWAQLCAQAGTGSPAPPADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALCRLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAPDPSPEAAFAAIERHGVTVTALVPALAKLWAQSCEWEPVTPKSLRLLQVGGSKLEPEDARRVRTALTPGLQQVFGMAEGLLNFTRIGDPPEVVEHTQGRPLCPADELRIVNADGEPVGPGEEGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSGDLVRRRDDGNLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSAAAVGLPDQYLGEKICAAVVFAGAPITLAELNGYLDRRGVAAHTRPDQLVAMPALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH
Bibliography
- Quadri LE et al. [1998]. Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Secondary Function
- De Voss JJ et al. [1999]. Iron acquisition and metabolism by mycobacteria. Review
- Silakowski B et al. [2000]. The myxochelin iron transport regulon of the myxobacterium Stigmatella aurantiaca Sg a15. Secondary Function
- Gold B et al. [2001]. The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Regulon
- Rodriguez GM, Voskuil MI, Gold B, Schoolnik GK and Smith I [2002]. ideR, An essential gene in mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
