Gene Rv2412
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in translation mechanisms. Binds directly to 16S ribosomal RNA. |
| Product | 30S ribosomal protein S20 RpsT |
| Comments | Rv2412, (MT2485, MTCY253.08c), len: 86 aa. rpsT, 30s ribosomal protein s20, equivalent to O33132|RS20_MYCLE|L0604|MLCL536.06 30S ribosomal protein S20 from Mycobacterium leprae (86 aa), FASTA scores: opt: 456, E(): 4.6e-24, (87.20% identity in 86 aa overlap). Also highly similar or similar to others e.g. Q9RDM3|RPST|SCC123.01 30S ribosomal protein S20 from Streptomyces coelicolor (88 aa), FASTA scores: opt: 363, E(): 7.1e-18, (70.95% identity in 86 aa overlap); Q9KD79|RPST|BH1339 ribosomal protein S20 (BS20) from Bacillus halodurans (91 aa), FASTA scores: opt: 252, E(): 1.8e-10, (49.4% identity in 85 aa overlap); P02378|RS20_ECOLI 30s ribosomal protein s20 from Escherichia coli (86 aa), FASTA scores: opt: 210, E(): 1e-07, (42.4% identity in 85 aa overlap); etc. Belongs to the S20P family of ribosomal proteins. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h of starvation (see citation below). |
| Mutant | essential gene by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2710075 | 2710335 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2412|rpsT
VANIKSQQKRNRTNERARLRNKAVKSSLRTAVRAFREAAHAGDKAKAAELLASTNRKLDKAASKGVIHKNQAANKKSALAQALNKL
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
