Gene Rv2458
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Catalyzes methyl transfer from S-methylmethionine or S-adenosylmethionine (less efficient) to homocysteine, selenohomocysteine and less efficiently selenocysteine [catalytic activity: S-adenosyl-L-methionine + L-homocysteine = S-adenosyl-L-homocysteine + L-methionine]. |
| Product | Probable homocysteine S-methyltransferase MmuM (S-methylmethionine:homocysteine methyltransferase) (cysteine methyltransferase) |
| Comments | Rv2458, (MTV008.14), len: 302 aa. Probable mmuM, homocysteine S-methyltransferase, equivalent to Q9CBY5|ML1478 possible transferase from Mycobacterium leprae (293 aa), FASTA scores: opt: 1507, E(): 2.7e-86, (78.85% identity in 293 aa overlap). Also similar to others e.g. Q47690|MMUM_ECOLI|B0261 homocysteine S-methyltransferase from Escherichia coli strain K12 (310 aa), FASTA scores: opt: 863, E(): 2.4e-46, (47.65% identity in 298 aa overlap); Q9FUM7 homocysteine S-methyltransferase-4 from Zea mays (Maize) (342 aa), FASTA scores: opt: 324, E(): 6.8e-13, (44.45% identity in 306 aa overlap); Q9LUI7|HMT3 cysteine methyltransferase from Arabidopsis thaliana (Mouse-ear cress) (347 aa), FASTA scores: opt: 312, E(): 3.8e-12, (41.85% identity in 313 aa overlap); etc. Identical to AAK46833|MT2533 homocysteine S-methyltransferase from Mycobacterium tuberculosis strain CDC1551 (302 aa). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2759779 | 2760687 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2458|mmuM
VELVSDSVLISDGGLATELEARGHDLSDPLWSARLLVDAPHAITAVHTAYFRAGAQIATTASYQASFEGFAARGIGHDDATVLLRRSVELAQAARDEVGVGGLSVAASVGPYGAALADGSEYRGYYGLSVAALMKWHLPRLEVLVDAGADMLALETIPDIDEAEALVNLVRRLATPAWLSYTINGTRTRAGQPLTDAFAVAAGVPEIVAVGVNCCAPDDVLPAIAFAVAHTGKPVIVYPNSGEGWDGRRRAWVGPRRFSGSSGQLAREWVAAGARIVGGCCRVRPIDIAEIGRALTTAPPRG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
