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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in the metabolism of PPGPP (at the first step). In eubacteria PPGPP (guanosine 3'-diphosphate 5-'diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of PPPGPP which is then hydrolysed to form PPGPP [catalytic activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5-'triphosphate].
ProductProbable GTP pyrophosphokinase RelA (ATP:GTP 3'- pyrophosphotransferase) (PPGPP synthetase I) ((P)PPGPP synthetase) (GTP diphosphokinase)
CommentsRv2583c, (MTCY227.18), len: 790 aa. Probable relA, GTP pyrophosphokinase, equivalent to Q49640|RELA_MYCLE|ML0491|MLCB1259.09|B1177_C1_168 probable GTP pyrophosphokinase from Mycobacterium leprae (787 aa), FASTA scores: opt: 4834, E(): 0, (93.4% identity in 790 aa overlap). Also highly similar to others e.g. O87331|RELA_CORGL|RELA|rel from Corynebacterium glutamicum (Brevibacterium flavum) (760 aa), FASTA scores: opt: 3375, E(): 1.6e-196, (67.0% identity in 758 aa overlap); O85709|RELA_STRAT from Streptomyces antibioticus (841 aa), FASTA scores: opt: 3209, E(): 1.9e-186, (63.85% identity in 786 aa overlap); Q9KDH1|RELA|BH1242 from Bacillus halodurans (728 aa), FASTA scores: opt: 2195,E(): 3.8e-125, (45.65% identity in 714 aa overlap); etc. Belongs to the RELA / spot family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tubeculosis H37Rv relA|Rv2583c mutant shows growth defect in vitro; can not grow at 42 degrees; has growth defect under starvation and under hypoxic conditions; growth in THP-1 macrophages is comparable to wild-type (See Primm et al., 2000); is attenuated in C57BL/6 mice (See Dahl et al., 2003).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS29078262910198-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2583c|relA
VAEDQLTAQAVAPPTEASAALEPALETPESPVETLKTSISASRRVRARLARRMTAQRSTTNPVLEPLVAVHREIYPKADLSILQRAYEVADQRHASQLRQSGDPYITHPLAVANILAELGMDTTTLVAALLHDTVEDTGYTLEALTEEFGEEVGHLVDGVTKLDRVVLGSAAEGETIRKMITAMARDPRVLVIKVADRLHNMRTMRFLPPEKQARKARETLEVIAPLAHRLGMASVKWELEDLSFAILHPKKYEEIVRLVAGRAPSRDTYLAKVRAEIVNTLTASKIKATVEGRPKHYWSIYQKMIVKGRDFDDIHDLVGVRILCDEIRDCYAAVGVVHSLWQPMAGRFKDYIAQPRYGVYQSLHTTVVGPEGKPLEVQIRTRDMHRTAEYGIAAHWRYKEAKGRNGVLHPHAAAEIDDMAWMRQLLDWQREAADPGEFLESLRYDLAVQEIFVFTPKGDVITLPTGSTPVDFAYAVHTEVGHRCIGARVNGRLVALERKLENGEVVEVFTSKAPNAGPSRDWQQFVVSPRAKTKIRQWFAKERREEALETGKDAMAREVRRGGLPLQRLVNGESMAAVARELHYADVSALYTAIGEGHVSAKHVVQRLLAELGGIDQAEEELAERSTPATMPRRPRSTDDVGVSVPGAPGVLTKLAKCCTPVPGDVIMGFVTRGGGVSVHRTDCTNAASLQQQAERIIEVLWAPSPSSVFLVAIQVEALDRHRLLSDVTRALADEKVNILSASVTTSGDRVAISRFTFEMGDPKHLGHLLNAVRNVEGVYDVYRVTSAA