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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in protein export. Part of the prokaryotic protein translocation apparatus which comprise SECA, SECB, SECD, SECE, SECF, SECG and SECY.
ProductProbable protein-export membrane protein SecF
CommentsRv2586c, (MT2663, MTCY227.15), len: 442 aa. Probable secF, protein-export membrane protein (integral membrane protein) (see citation below), equivalent to P38386|SECF_MYCLE|SECF|ML0488|MLCB1259.06|B1177_C3_239 protein-export membrane protein from Mycobacterium leprae (471 aa), FASTA scores: opt: 1910, E(): 2.9e-104, (72.15% identity in 456 aa overlap). Also similar to others e.g. Q9AE06|SECF from Corynebacterium glutamicum (Brevibacterium flavum) (403 aa), FASTA scores: opt: 1198, E(): 9.8e-63, (47.1% identity in 399 aa overlap); Q53956|SECF_STRCO|SCL2.05c from Streptomyces coelicolor (373 aa), FASTA scores: opt: 670, E(): 6.4e-32, (39.25% identity in 400 aa overlap); Q55611|SECF_SYNY3|SLR0775 from Synechocystis sp. strain PCC 6803 (315 aa), FASTA scores: opt: 416, E(): 3.8e-17, (33.8% identity in 296 aa overlap); etc. Belongs to the SECD/SECF family, SECF family. Part of the prokaryotic protein translocation apparatus which comprise SECA|Rv3240c, SECD|Rv2587c, SECE|Rv0638, SECF, SECG|Rv1440 and SECY|Rv0732.
Functional categoryCell wall and cell processes
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS; predicted transmembrane protein (See Gu et al., 2003). Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantEssential gene domain for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS29126832914011-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2586c|secF
MASKAKTGRDDEATSAVELTEATESAVARTDGDSTTDTASKLGHHSFLSRLYTGTGAFEVVGRRRLWFGVSGAIVAVAIASIVFRGFTFGIDFKGGTTVSFPRGSTQVAQVEDVYYRALGSEPQSVVIVGAGASATVQIRSETLTSDQTAKLRDALFEAFGPKGTDGQPSKQAISDSAVSETWGGQITKKAVIALVVFLVLVALYITVRYERYMTISAITAMLFDLTVTAGVYSLVGFEVTPATVIGLLTILGFSLYDTVIVFDKVEENTHGFQHTTRRTFAEQANLAINQTFMRSINTSLIGVLPVLALMVVAVWLLGVGTLKDLALVQLIGIIIGTYSSIFFATPLLVTLRERTELVRNHTRRVLKRRNSGSPAGSEDASTDGGEQPAAADEQSLVGITQASSQSAPRAAQGSSKPAPGARPVRPVGTRRPTGKRNAGRR