Gene Rv2681
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical alanine rich protein |
| Comments | Rv2681, (MTCY05A6.02), len: 438 aa. Conserved hypothetical ala-rich protein, equivalent to Q50004|ML1040|U1764U hypothetical protein from Mycobacterium leprae (429 aa), FASTA scores: opt: 2146, E(): 1.1e-119, (77.4% identity in 416 aa overlap). Also highly similar to O69858|SC1C3.16c hypothetical 42.5 KDA protein from Streptomyces coelicolor (394 aa), FASTA scores: opt: 1336, E(): 9e-72, (51.6% identity in 405 aa overlap); and with some similarity to ribonucleases D e.g. Q983F2|MLL8354 from Rhizobium loti (Mesorhizobium loti) (383 aa), FASTA scores: opt: 379, E(): 3.9e-15, (31.6% identity in 323 aa overlap); Q9A7L8|CC1704 from Caulobacter crescentus (389 aa), FASTA scores: opt: 370, E(): 1.3e-14, (31.45% identity in 318 aa overlap); CAC45770 from Rhizobium meliloti (Sinorhizobium meliloti) (383 aa), FASTA scores: opt: 331, E(): 2.7e-12, (27.75% identity in 357 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Slow growth mutant by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2996739 | 2998055 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2681|Rv2681
MCPEPSHAGAAESEGTESEPTPLLRPAGGIPDLCVTVGEIAAAAELLDRGRGPFAVDAERASGFRYSGRAYLIQIRRAEAGTVLIDPVSHGGDPLTVLAPVAEVLSTNEWILHSADQDLPCLAEVGMRPPALYDTELAGRLAGFDRVNLAAMVERLLGLGLTKGHGAADWSKRPLPSAWLNYAALDVELLIELRAAISRVLAEQGKTDWAAQEFEHLRSFESRPPPAAARQDRWRRTSGIHKVHDRRGLAAVRELWTARDRIAQRRDIAPRRILPDSAIIDAAIADPKSVDDLVALPVFGGRNQRRSAAVWWAALAAARESPDPPEIAEPANGPPPPGRWVRRKPAAAARLDAARAALTEVSQRVRVPTENLVSPDLVRRLCWEWEDISQSSPDPIAAVEAYLRTGQARAWQLELVVPILTAALTGAPDAGAQGDDGS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
