Gene Rv2701c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in inositol phosphate metabolism. It is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. Key enzyme of the phosphatidyl inositol signaling pathway. [catalytic activity: myo-inositol 1-phosphate + H(2)O = myo-inositol + phosphate] |
| Product | Inositol-1-monophosphatase SuhB |
| Comments | Rv2701c, (MTCY05A6.22c), len: 290 aa. SuhB, inositol-1-monophosphatase. Equivalent to AAK47090 from Mycobacterium tuberculosis strain CDC1551 (277 aa) but longer 13 aa. Contains PS00630 Inositol monophosphatase family signatures 1 and 2 (PS00629 and PS00630). Belongs to the inositol monophosphatase family. Cofactor: Mg2+. Activity is inhibited by Li+ but not when Leu81 is mutated (See Nigou et al., 2002). Mg2+ promotes dimerization; Li+ amplifies this effect but does not promote dimerization on its own (See Brown et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Colony morphology of M. tuberculosis H37Rv suhB|Rv2701c deletion mutant is not different from wild-type; phosphatidylinositol mannosides (PIMs; by TLC) and mycothiol levels (by HPLC) are normal in the mutant (See Movahedzadeh et al., 2010). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3015863 | 3016735 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2701c|suhB
VTRPDNEPARLRSVAENLAAEAAAFVRGRRAEVFGISRAGDGDGAVRAKSSPTDPVTVVDTDTERLLRDRLAQLRPGDPILGEEGGGPADVTATPSDRVTWVLDPIDGTVNFVYGIPAYAVSIGAQVGGITVAGAVADVAARTVYSAATGLGAHLTDERGRHVLRCTGVDELSMALLGTGFGYSVRCREKQAELLAHVVPLVRDVRRIGSAALDLCMVAAGRLDAYYEHGVQVWDCAAGALIAAEAGARVLLSTPRAGGAGLVVVAAAPGIADELLAALQRFNGLEPIPD
Bibliography
- Nigou J et al. [2002]. Purification and biochemical characterization of Mycobacterium tuberculosis SuhB, an inositol monophosphatase involved in inositol biosynthesis. Biochemistry Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Brown AK et al. [2007]. Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg2+. Biochemistry Structure
- Movahedzadeh F et al. [2010]. Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
