Go to browser
virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionThe sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released. This is the primary sigma-factor of this bacteria. Supposedly involved in the housekeeping regulons.
ProductRNA polymerase sigma factor SigA (sigma-A)
CommentsRv2703, (MTCY05A6.24), len: 528 aa. SigA (formerly named mysA, and also known as rpoV or rpoD), RNA polymerase sigma factor (see citations below), equivalent (but shorter 55 aa) to Q9S5K3|RPOT (alias Q59532) RNA polymerase sigma factor from Mycobacterium leprae (576 aa), FASTA scores: opt: 2638, E(): 8.6e-115, (80.35% identity in 535 aa overlap). Also similar to others e.g. Q59552|MYSA from Mycobacterium smegmatis (466 aa), FASTA scores: opt: 2259, E(): 2.3e-97, (76.5% identity in 528 aa overlap); Q45302|SIGA from Corynebacterium glutamicum (Brevibacterium flavum) (497 aa), FASTA scores: opt: 1972, E(): 4.3e-84, (67.35% identity in 505 aa overlap); Q59813|HRDB from Streptomyces aureofaciens (525 aa), FASTA scores: opt: 1654, E(): 2.1e-69, (67.5% identity in 468 aa overlap); etc. Contains sigma-70 family signatures 1 and 2 (PS00715 and PS00716). Belongs to the sigma-70 factor family.
Functional categoryInformation pathways
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
TranscriptomicsmRNA identified by SCOTS method, during infection of cultured human primary macrophages (see Graham & Clark-Curtiss 1999). mRNA also identified by real-time quantitative RT-PCR during exponential growing cultures (see Manganelli et al., 1999) and in stationary-phase bacteria (see Hu et al., 2000).
MutantEssential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2703|sigA