Gene Rv2763c (folA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP [catalytic activity: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH]. |
| Product | Dihydrofolate reductase DfrA (DHFR) (tetrahydrofolate dehydrogenase) |
| Comments | Rv2763c, (MTV002.28c), len: 159 aa. Probable dfrA (alternate gene names: folA, dhfr), dihydrofolate reductase, equivalent to O30463|FOLA dihydrofolate reductase from Mycobacterium avium (see citation below) (181 aa), FASTA scores: opt: 802, E(): 4.5e-48, (70.2% identity in 161 aa overlap); and Q9CBW1|FOLA|ML1518 dihydrofolate reductase from Mycobacterium leprae (165 aa), FASTA scores: opt: 782, E(): 1e-46, (70.55% identity in 163 aa overlap). Also highly similar to many e.g. Q9K168|DYR_NEIMB|FOLA|NMB0308 from Neisseria meningitidis (serogroup B) (162 aa), FASTA scores: opt: 469, E(): 3.8e-25, (46.65% identity in 163 aa overlap); P12833|DYR3_SALTY|DHFRIII from Salmonella typhimurium (162 aa), FASTA scores: opt: 367, E(): 4e-18, (45.4% identity in 141 aa overlap); Q59408|DYRC_ECOLI|DHFRXIII from Escherichia coli strain RA33.2 (165 aa), FASTA scores: opt: 313, E(): 2.2e-14, (41.9% identity in 136 aa overlap); etc. Contains PS00075 Dihydrofolate reductase signature. Belongs to the dihydrofolate reductase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3073130 | 3073609 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2763c|dfrA
MVGLIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNVVLSRQADFMASGAEVVGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLDETWRGETGEWRFSRSGLRYRLYSYHRS
Bibliography
- Zywno-van Ginkel S et al. [1997]. Identification and cloning of the Mycobacterium avium folA gene, required for dihydrofolate reductase activity. Sequence Product
- Li R et al. [2000]. Three-dimensional structure of M. tuberculosis dihydrofolate reductase reveals opportunities for the design of novel tuberculosis drugs. Product Structure
- Argyrou A et al. [2006]. Mycobacterium tuberculosis dihydrofolate reductase is a target for isoniazid. Structure
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
