Gene Rv2793c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Formation of pseudouridine at position 55 in the psi GC loop of transfer RNAS [catalytic activity: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O]. |
| Product | Probable tRNA pseudouridine synthase B TruB (tRNA pseudouridine 55 synthase) (PSI55 synthase) (pseudouridylate synthase) (uracil hydrolyase) |
| Comments | Rv2793c, (MTV002.58c), len: 298 aa. Probable truB, tRNA pseudouridine synthase, equivalent to Q9Z5I4|TRUB_MYCLE|ML1546 or MLCB596.24 tRNA pseudouridine synthase B from Mycobacterium leprae (320 aa), FASTA scores: opt: 1403, E(): 2.9e-83, (74.05% identity in 293 aa overlap). Also highly similar to many e.g. Q9Z528|TRUB_STRCO|SC9F2.07c from Streptomyces coelicolor (301 aa), FASTA scores: opt: 870, E(): 7.6e-49, (50.7% identity in 296 aa overlap); P09171|TRUB_ECOLI|P35|B3166|Z4527|ECS4047 from Escherichia coli strains K12 and O157:H7 (314 aa), FASTA scores: opt: 574, E(): 1e-29, (42.5% identity in 214 aa overlap); Q9PGR1|TRUB_XYLFA|XF0237 from Xylella fastidiosa (302 aa), FASTA scores: opt: 569, E(): 2.1e-29, (41.05% identity in 285 aa overlap); etc. Belongs to the TruB family of pseudouridine synthases. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3102364 | 3103260 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2793c|truB
MSATGPGIVVIDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVIGIERATKILGLLTAAPKSYAATIRLGQTTSTEDAEGQVLQSVPAKHLTIEAIDAAMERLRGEIRQVPSSVSAIKVGGRRAYRLARQGRSVQLEARPIRIDRFELLAARRRDQLIDIDVEIDCSSGTYIRALARDLGDALGVGGHVTALRRTRVGRFELDQARSLDDLAERPALSLSLDEACLLMFARRDLTAAEASAAANGRSLPAVGIDGVYAACDADGRVIALLRDEGSRTRSVAVLRPATMHPG
Bibliography
- Chaudhuri BN et al. [2004]. Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft. Structure
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
