Gene Rv2902c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Degrades the ribonucleotide moiety on RNA-DNA hybrid molecules [catalytic activity: endonucleolytic cleavage to 5'- phosphomonoester]. |
| Product | Probable ribonuclease HII protein RnhB (RNase HII) |
| Comments | Rv2902c, (MT2970, MTCY274.33c), len: 264 aa. Probable rnhB, ribonuclease HII, equivalent to O33022|RNH2_MYCLE|RNHB|ML1611|MLCB250.40 ribonuclease HII from Mycobacterium leprae (240 aa), FASTA scores: opt: 1242, E(): 6.9e-72, (76.75% identity in 245 aa overlap). Also similar (but longer ~20 aa) to others e.g. Q9HXY9|RNHB|PA3642 ribonuclease HII from Pseudomonas aeruginosa (201 aa), FASTA scores: opt: 572, E(): 3.1e-29, (52.7% identity in 184 aa overlap); Q9PEI7|RNH2_XYLFA|RNHB|XF1041 ribonuclease HII from Xylella fastidiosa (234 aa), FASTA scores: opt: 556, E(): 3.6e-28, (50.25% identity in 185 aa overlap); P10442|RNH2_ECOLI|RNHB|B0183 ribonuclease HII from Escherichia coli strain K-12 (213 aa), FASTA scores: opt: 519, E(): 7.4e-26, (48.65% identity in 183 aa overlap); etc. Belongs to the RNASE HII family. Cofactor: manganese (by similarity). |
| Functional category | Information pathways |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3212162 | 3212956 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2902c|rnhB
MTKTWPPRTVIRKSGGLRGMRTLESALHRGGLGPVAGVDEVGRGACAGPLVVAACVLGPGRIASLAALDDSKKLSEQAREKLFPLICRYAVAYHVVFIPSAEVDRRGVHVANIEGMRRAVAGLAVRPGYVLSDGFRVPGLPMPSLPVIGGDAAAACIAAASVLAKVSRDRVMVALDADHPGYGFAEHKGYSTPAHSRALARLGPCPQHRYSFINVRRVASGSNTAEVADGQPDPRDGTAQTGEGRWSKSSHPATMRATGRAQGT
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
