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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionInvolved in leucine biosynthesis (at the third step) [catalytic activity: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH (the product decarboxylates to 4-methyl-2-oxopentanoate)].
ProductProbable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
CommentsRv2995c, (MTV012.09), len: 336 aa. Probable leuB, 3-isopropylmalate dehydrogenase, identical except a single bp to P94929|LEU3_MYCBO 3-isopropylmalate dehydrogenase from Mycobacterium bovis (336 aa) (see citation below), FASTA scores: opt: 2168, E(): 5.1e-132, (99.7% identity in 336 aa overlap); and equivalent to O33117|LEU3_MYCLE 3-isopropylmalate dehydrogenase from Mycobacterium leprae (336 aa), FASTA scores: opt: 1864, E(): 1.8e-112, (83.95% identity in 336 aa overlap). Also highly similar to others e.g. P94631|LEU3_CORGL from Corynebacterium glutamicum (340 aa), FASTA scores: opt: 1526, E(): 1e-90, (69.9% identity in 339 aa overlap); O86504 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 1470, E(): 4.2e-87, (67.85% identity in 339 aa overlap); Q9UZ05|PAB2424 from Pyrococcus abyssi (354 aa), FASTA scores: opt: 998, E(): 1e-56, (50.0% identity in 322 aa overlap); etc. Note that also shows high similarity with many tartrate dehydrogenases. Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2995c|leuB