Gene Rv2995c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in leucine biosynthesis (at the third step) [catalytic activity: 3-carboxy-2-hydroxy-4-methylpentanoate + NAD(+) = 3-carboxy-4-methyl-2-oxopentanoate + NADH (the product decarboxylates to 4-methyl-2-oxopentanoate)]. |
| Product | Probable 3-isopropylmalate dehydrogenase LeuB (beta-IPM dehydrogenase) (IMDH) (3-IPM-DH) |
| Comments | Rv2995c, (MTV012.09), len: 336 aa. Probable leuB, 3-isopropylmalate dehydrogenase, identical except a single bp to P94929|LEU3_MYCBO 3-isopropylmalate dehydrogenase from Mycobacterium bovis (336 aa) (see citation below), FASTA scores: opt: 2168, E(): 5.1e-132, (99.7% identity in 336 aa overlap); and equivalent to O33117|LEU3_MYCLE 3-isopropylmalate dehydrogenase from Mycobacterium leprae (336 aa), FASTA scores: opt: 1864, E(): 1.8e-112, (83.95% identity in 336 aa overlap). Also highly similar to others e.g. P94631|LEU3_CORGL from Corynebacterium glutamicum (340 aa), FASTA scores: opt: 1526, E(): 1e-90, (69.9% identity in 339 aa overlap); O86504 from Streptomyces coelicolor (347 aa), FASTA scores: opt: 1470, E(): 4.2e-87, (67.85% identity in 339 aa overlap); Q9UZ05|PAB2424 from Pyrococcus abyssi (354 aa), FASTA scores: opt: 998, E(): 1e-56, (50.0% identity in 322 aa overlap); etc. Note that also shows high similarity with many tartrate dehydrogenases. Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3352458 | 3353468 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2995c|leuB
MKLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNHDAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVVREGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTNVLTFAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDLAAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLGEHDAAARVDRAVEAHLATRGSERLATSDVGERIAAAL
Bibliography
- Han MY et al. [1997]. Molecular cloning of the leuB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis. Sequence
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Singh RK et al. [2005]. The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis. Structure
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Mueller-Dieckmann C et al. [2007]. On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths. Structure
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
