Gene Rv3009c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Component of the translational apparatus. Furnishes a means for formation of correctly charged GLN-tRNA(GLN) through the transamidation of misacylated GLU- tRNA(GLN) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-GLU-tRNA(GLN) [catalytic activity: ATP + L-glutamyl-tRNA(GLN) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(GLN) + L-glutamate]. |
| Product | Probable glutamyl-tRNA(GLN) amidotransferase (subunit B) GatB (Glu-ADT subunit B) |
| Comments | Rv3009c, (MT3089, MTV012.23c), len: 509 aa. Probable gatB, Glu- tRNA-Gln amidotransferase, subunit B , equivalent to O33107|GATB_MYCLE|MLCB637_15 glutamyl-tRNA(GLN) amidotransferase from Mycobacterium leprae (509 aa), FASTA scores: opt: 2973, E(): 2.9e-173, (88.4% identity in 509 aa overlap). Also highly similar to other Glu- tRNA-Gln amidotransferases e.g. Q9Z578|GATB|SC8D9.13 from Streptomyces coelicolor (504 aa), FASTA scores: opt: 2264, E(): 3.6e-130, (66.0% identity in 495 aa overlap); P74215|GATB_SYNY3|SLL1435 from Synechocystis sp. strain PCC 6803 (519 aa), FASTA scores: opt: 1289, E(): 6.7e-71, (42.0% identity in 485 aa overlap); Q9X100|GATB_THEMA|TM1273 glutamyl-tRNA(GLN) amidotransferase from Thermotoga maritima (482 aa), FASTA scores: opt: 1165, E(): 2.2e-63, (40.05% identity in 487 aa overlap); etc. For more information about function, see citation below. Similar to many members of the pet112 family. Belongs to the GatB family. |
| Functional category | Information pathways |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3367264 | 3368793 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3009c|gatB
MTVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVCPVCLGLPGSLPVLNRAAVESAIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSETGRIHGATGSLIDYNRAGVPLIEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTEFGTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAEDYRYFPEPDLEPVAPSRELVERLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVELVAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITPAQVAAVVALVDEGKLSNSLARQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGAIVGAVMKATRGQADAARVRELVLEACGQG
Bibliography
- Curnow AW et al. [1997]. Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation. Homolog Product Function Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
