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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in translation mechanisms [catalytic activity: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate].
ProductProbable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU
CommentsRv3024c, (MT3108, MTV012.39c), len: 367 aa. Probable trmU, tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase , equivalent to O33099|TRMU_MYCLE|ML1707|MLCB637.07 probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase from Mycobacterium leprae (358 aa), FASTA scores: opt: 2033, E(): 5.5e-116, (85.45% identity in 357 aa overlap). Also highly similar to others e.g. O86583|TRMU_STRCO|SC2A11.22 from Streptomyces coelicolor (376 aa), FASTA scores: opt: 1336, E(): 1e-73, (56.9% identity in 369 aa overlap); BAB49856|MLR2824 from Rhizobium loti (378 aa), FASTA scores: opt: 826, E(): 8.3e-43, (42.35% identity in 359 aa overlap); Q9ZDM1|TRMU_RICPR|RP306 from Rickettsia prowazekii (358 aa), FASTA scores: opt: 800, E(): 3e-41, (40.1% identity in 359 aa overlap); etc. Belongs to the TrmU family.
Functional categoryInformation pathways
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS33827853383888-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3024c|trmU
MKVLAAMSGGVDSSVAAARMVDAGHEVVGVHMALSTAPGTLRTGSRGCCSKEDAADARRVADVLGIPFYVWDFAEKFKEDVINDFVSSYARGETPNPCVRCNQQIKFAALSARAVALGFDTVATGHYARLSGGRLRRAVDRDKDQSYVLAVLTAQQLRHAAFPIGDTPKRQIRAEAARRGLAVANKPDSHDICFIPSGNTKAFLGERIGVRRGVVVDADGVVLASHDGVHGFTIGQRRGLGIAGPGPNGRPRYVTAIDADTATVHVGDVTDLDVQTLTGRAPVFTAGAAPSGPVDCVVQVRAHGETVSAVAELIGDALFVQLHAPLRGVARGQTLVLYRPDPAGDEVLGSATIAGASGLSTGGNPGA