Gene Rv3057c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown, but similar to various oxidoreductases and enzymes involved in polyketides synthesis |
| Product | Probable short chain alcohol dehydrogenase/reductase |
| Comments | Rv3057c, (MTCY22D7.24), len: 287 aa. Probable oxidoreductase, probably short-chain alcohol dehydrogenase/reductase. Equivalent to Q9CBP7|ML1740 possible short chain dehydrogenases/reductase from Mycobacterium leprae (312 aa), FASTA scores: opt: 1563, E(): 6e-89, (81.8% identity in 280 aa overlap). Also similar to many oxidoreductases e.g. Q9ZBX8|SCD78.21c putative oxidoreductase from Streptomyces coelicolor (585 aa), FASTA scores: opt: 541, E(): 6.7e-26, (37.25% identity in 263 aa overlap); AAK47506|MT3170 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 521, E(): 6.1e-25, (36.25% identity in 276 aa overlap); AAK45541|MT1283 oxidoreductase, short-chain dehydrogenase/reductase family from Mycobacterium tuberculosis strain CDC1551 (276 aa), FASTA scores: opt: 471, E(): 7.2e-22, (32.4% identity in 281 aa overlap). Also similar to O50460|Rv1245c|MTV006.17C dehydrogenase (276 aa). Contains short-chain alcohol dehydrogenase family signature (PS00061). May belong to the short-chain dehydrogenases/reductases (SDR) family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3417799 | 3418662 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3057c|Rv3057c
MLQRGAGQYFAGKRCFVTGAASGIGRATALRLAAQGAELYLTDRDRDGLAQTVCDARALGAQVPEHRVLDVSDYQDVAAFAADIHARHPSMDVVLNIAGVSAWGTVDQLTHDQWSRMVAINLMGPIHVIETLVPPMVAAGRGGHLVNVSSAAGLVGLPWHAAYSASKYGLRGLSEVLRFDLARHGIGVSVVVPGAVKTPLVNTVEIAGVDRDDPRVNRWVERFSGHAVTPEKAADKILAGVTRNRYLVYTSADIRALYAFKRYAWWPYTLVMRRVNVFFTRALRPGP
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
