Gene Rv3124
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Transcriptional regulatory protein MoaR1 |
| Comments | Rv3124, (MTCY164.34), len: 289 aa. MoaR1, transcriptional regulatory protein, similar to many Streptomyces and Mycobacterium tuberculosis regulatory proteins e.g. Q11052|YC67_MYCTU|Rv1267c|MT1305|MTCY50.15 from Mycobacterium tuberculosis strain H37Rv (388 aa), FASTA scores: opt: 963, E(): 2e-56, (55.15% identity in 252 aa overlap); O53145 from Mycobacterium tuberculosis (381 aa); P71484|EMBR from Mycobacterium avium (384 aa), FASTA scores: opt: 859, E(): 1.5e-49, (52.2% identity in 249 aa overlap); Q9XCC3|TYLT from Streptomyces fradiae (404 aa), FASTA scores: opt: 462, E(): 3.1e-23, (35.05% identity in 254 aa overlap); Q9XCC4|TYLS from Streptomyces fradiae (277 aa), FASTA scores: opt: 456, E(): 5.6e-23, (33.45% identity in 269 aa overlap); etc. Start chosen by similarity, alternative possible (see AAK47548 from Mycobacterium tuberculosis strain CDC1551, longer N-terminus (311 aa)). This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Regulatory proteins |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3489506 | 3490375 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3124|moaR1
VQFNVLGPLELNLRGTKLPLGTPKQRAVLAMLLLSRNQVVAADALVQAIWEKSPPARARRTVHTYICNLRRTLSDAGVDSRNILVSEPPGYRLLIGDRQQCDLDRFVAAKESGLRASAKGYFSEAIRYLDSALQNWRGPVLGDLRSFMFVQMFSRALTEDELLVHTKLAEAAIACGRADVVIPKLERLVAMHPYRESLWKQLMLGYYVNEYQSAAIDAYHRLKSTLAEELGVEPAPTIRALYHKILRQLPMDDLVGRVTRGRVDLRGGNGAKVEELTESDKDLLPIGLA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Mendoza Lopez P et al. [2010]. Characterization of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a non-synonymous SNP in the Mycobacterium bovis BCG orthologue. Function Product
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
