Gene Rv3160c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in transcriptional mechanism. |
| Product | Possible transcriptional regulatory protein (probably TetR-family) |
| Comments | Rv3160c, (MTV014.04c), len: 213 aa. Possible transcriptional regulator, with some similarity to others e.g. Q9S3L4|AMTR AMTR protein (global repressor in the nitrogen regulation system; see Jakoby et al., 2000) (222 aa), FASTA scores: opt: 182, E(): 7.3e-05, (27.9% identity in 208 aa overlap); Q9X7X9|SC6A5.33c putative regulatory protein from Streptomyces coelicolor (223 aa), FASTA scores: opt: 176, E(): 0.00018, (26.5% identity in 185 aa overlap); Q9XA31|SCH69.03c putative transcriptional regulator from Streptomyces coelicolor (209 aa), FASTA scores: opt: 173, E(): 0.00027, (27.25% identity in 176 aa overlap); BAB54133|MLL7734 transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (213 aa), FASTA scores: opt: 172, E(): 0.00031, (23.55% identity in 204 aa overlap); etc. Also similar to hypothetical proteins from Mycobacterium tuberculosis strain H37Rv e.g. P96839|Rv3557v|MTCY06G11.04c (200 aa), FASTA scores: opt: 169, E(): 0.00046, (26.75% identity in 157 aa overlap). Contains probable helix-turn-helix motif from aa 31 to 52 (Score 1857, +5.51 SD). Similar to the TetR/AcrR family of transcriptional regulators. |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h, 24h and 96h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3529338 | 3529979 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3160c|Rv3160c
MPRQAGRWSPTALRILGAAAELIALRGYSSTSTRDIAAAVGVEQPAIYKHFSAKRDILAALVRLAVEWPLELFGHITAMPVPAVVKLHRWLTESLDHLHASPYVLVSILITPDLHQESFVAERELVAEMERALVGLIETGQGEGDVRAMHPLSAARLVQALFDALALPEFAVSPDEIVEFAMTALLSDPDRLAEIRAAADALEIQTAPPDRGL
Bibliography
- Jakoby M et al. [2000]. AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum. Homolog Regulation
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
