Gene Rv3215
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Could be involved in enterobactin biosynthesis. Enterobactin is an iron-chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm. Could be also involved in 2,3-dihydroxybenzoate or enterochelin or menaquinone biosynthesis [catalytic activity: chorismate = isochorismate]. |
| Product | Probable isochorismate synthase EntC (isochorismate hydroxymutase) (enterochelin biosynthesis) |
| Comments | Rv3215, (MTCY07D11.11c), len: 372 aa. Probable entC, isochorismate synthase, equivalent to Q9CCH5|ENTC|ML0808 putative isochorismate synthase from Mycobacterium leprae (577 aa), FASTA scores: opt: 1817, E(): 5.5e-105, (73.5% identity in 366 aa overlap). Also similar to others e.g. Q9F639|MXCD protein involved in myxochelin-type iron chelator biosynthesis (see citation below) from Stigmatella aurantiaca (408 aa), FASTA scores: opt: 893, E(): 6.2e-48, (41.6% identity in 382 aa overlap); P45744|DHBC_BACSU isochorismate synthase from Bacillus subtilis (398 aa), FASTA scores: opt: 883, E(): 2.5e-47, (40.45% identity in 393 aa overlap); Q9KI93|CSBC isochorismate synthase (fragment) from Azotobacter vinelandii (361 aa), FASTA scores: opt: 794, E(): 7.6e-42, (45.65% identity in 298 aa overlap); and the two Escherichia coli proteins AAG54928|ENTC (alias BAB34055|ECS0632) isochorismate hydroxymutase 2 from Escherichia coli strain O157:H7 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); P10377|ENTC|B0593 isochorismate synthase from Escherichia coli strain K12 (391 aa), FASTA scores: opt: 744, E(): 1e-38, (38.8% identity in 340 aa overlap); etc. Stronger similarity to Escherichia coli entC. Also similar to MTCY253.35. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3592254 | 3593372 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3215|entC
VSAHVATLHPEPPFALCGPRGTLIARGVRTRYCDVRAAQAALRSGTAPILLGALPFDVSRPAALMVPDGVLRARKLPDWPTGPLPKVRVAAALPPPADYLTRIGRARDLLAAFDGPLHKVVLARAVQLTADAPLDARVLLRRLVVADPTAYGYLVDLTSAGNDDTGAALVGASPELLVARSGNRVMCKPFAGSAPRAADPKLDAANAAALASSAKNRHEHQLVVDTMRVALEPLCEDLTIPAQPQLNRTAAVWHLCTAITGRLRNISTTAIDLALALHPTPAVGGVPTKAATELIAELEGDRGFYAGAVGWCDGRGDGHWVVSIRCAQLSADRRAALAHAGGGIVAESDPDDELEETTTKFATILTALGVEQ
Bibliography
- Silakowski B et al. [2000]. The myxochelin iron transport regulon of the myxobacterium Stigmatella aurantiaca Sg a15. Secondary Function
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
