Gene Rv3220c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Sensor part of a two component regulatory system. |
| Product | Probable two component sensor kinase |
| Comments | Rv3220c, (MTCY07D11.06), len: 501 aa. Probable sensor (probably histidine kinase), equivalent to Q9CCH8|ML0803 putative two-component system sensor kinase from Mycobacterium leprae (500 aa). Similar to others e.g. Q9F3M1|2SC7G11.01 putative histidine kinase (fragment) from Streptomyces coelicolor (372 aa), FASTA scores: opt: 1038, E(): 7.4e-56, (48.95% identity in 380 aa overlap); Q9A3K5|CC3198 sensor histidine kinase from Caulobacter crescentus (327 aa), FASTA scores: opt: 311, E(): 1.2e-11, (33.35% identity in 201 aa overlap) (similarity only in C-terminal part for this one); Q9A2T2|CC3474 putative sensor histidine kinase from Caulobacter crescentus (547 aa); etc. C-terminal half shows similarity to many sensor proteins, that respond to various stimuli from Methanobacterium thermoautotrophicum e.g. O26568|MTH468 sensory transduction histidine kinase (554 aa), FASTA scores: opt: 425, E(): 2.1e-18, (34.0% identity in 244 aa overlap); O26546|MTH446 sensory transduction regulatory protein (583 aa), FASTA scores: opt: 380, E(): 1.2e-15, (37.15% identity in 202 aa overlap); O26913|MTH823 sensory transduction regulatory protein (677 aa), FASTA scores: opt: 375, E(): 2.7e-15, (35.4% identity in 195 aa overlap); etc. Seems similar to other prokaryotic sensory transduction histidine kinases. |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Virulence of M. tuberculosis H37Rv mutant in SCID mice is unchanged (See Parish et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3596029 | 3597534 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3220c|Rv3220c
MSTLGDLLAEHTVLPGSAVDHLHAVVGEWQLLADLSFADYLMWVRRDDGVLVCVAQCRPNTGPTVVHTDAVGTVVAANSMPLVAATFSGGVPGREGAVGQQNSCQHDGHSVEVSPVRFGDQVVAVLTRHQPELAARRRSGHLETAYRLCATDLLRMLAEGTFPDAGDVAMSRSSPRAGDGFIRLDVDGVVSYASPNALSAYHRMGLTTELEGVNLIDATRPLISDPFEAHEVDEHVQDLLAGDGKGMRMEVDAGGATVLLRTLPLVVAGRNVGAAILIRDVTEVKRRDRALISKDATIREIHHRVKNNLQTVAALLRLQARRTSNAEGREALIESVRRVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRINRVGDLGVLDSDRATALIMVITELVQNAIEHAFDPAAAEGSVTIRAERSARWLDVVVHDDGLGLPQGFSLEKSDSLGLQIVRTLVSAELDGSLGMRDARERGTDVVLRVPVGRRGRLML
Bibliography
- Parish T et al. [2003]. Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. Mutant Secondary Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
