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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in the hydrocarbon hydroxylating system to convert conversion of dodecane to lauric acid, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase.
ProductProbable rubredoxin RubB
CommentsRv3250c, (MTCY20B11.25c), len: 60 aa. Probable rubB, rubredoxin, highly similar to other rubredoxins e.g. Q9AE66|RUBA4 from Rhodococcus erythropolis (60 aa), FASTA scores: opt: 391, E(): 2.2e-21, (83.05% identity in 59 aa overlap); Q9AE63|RUBA2 from Rhodococcus erythropolis (63 aa), FASTA scores: opt: 380, E(): 1.4e-20, (83.9% identity in 56 aa overlap); P42453|RUBR_ACICA|RUBA from Acinetobacter calcoaceticus (54 aa), FASTA scores: opt: 315, E(): 4.9e-16, (69.8% identity in 53 aa overlap); Q9HTK7|PA5351 from Pseudomonas aeruginosa (55 aa), FASTA scores: opt: 298, E(): 8e-15, (64.15% identity in 53 aa overlap); Q9PGC3|XF0379 from Xylella fastidiosa (57 aa), FASTA scores: opt: 263, E(): 2.5e-12, (59.25% identity in 54 aa overlap); etc. Also similar to neighbouring ORF M. tuberculosis RubA (MTCY20B11.26c). Contains rubredoxin signature (PS00202). Belongs to the rubredoxin family.
Functional categoryIntermediary metabolism and respiration
TranscriptomicsmRNA identified by microarray analysis; transcription repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see citation below).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS36303843630566-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3250c|rubB
VNDYKLFRCIQCGFEYDEALGWPEDGIAAGTRWDDIPDDWSCPDCGAAKSDFEMVEVARS