Gene Rv3265c (wbbL)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Probably involved in cell wall arabinogalactan linker formation: Uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. Seems to be essential for mycobacterial viability. |
| Product | dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase WbbL1 (alpha-L-rhamnose-(1->3)-alpha-D-GlcNAc(1->P)-P-decaprenyl) |
| Comments | Rv3265c, (MTCY71.05c), len: 301 aa. wbbL1, dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol a-3-L-rhamnosyl transferase (see citations below), equivalent to Q9CCK7|WBBL|ML0752 putative dTDP-rhamnosyl transferase from Mycobacterium leprae (308 aa), FASTA scores: opt: 1788, E(): 3e-104, (85.05% identity in 301 aa overlap); and Q9RN50|WBBL|Q9RN49 (see note * below) dTDP-RHA:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase from Mycobacterium smegmatis (296 aa), FASTA scores: opt: 1494, E(): 6.1e-86, (72.35% identity in 293 aa overlap). Note that previously known as wbbL. [* Note: unpublished (experimental study on Mycobacterium smegmatis). Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases - The cell wall arabinogalactan linker formation enzyme, dTDP-Rha:a-D-GlcNAc-diphosphoryl polyprenol, a-3-L-rhamnosyl transferase is essential for mycobacterial viability - Mills J.A., Motichka K., Jucker M., Wu H.P., Uhlic B.C., Stern R.J., Scherman M.S., Vissa V.D., Yan W., Pan F., Kimbrel S., Kundu M., McNeil M.]. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | Note that corresponding mRNA identified in Mycobacterium smegmatis YM202 (see Ma et al., 2002). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3645979 | 3646884 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3265c|wbbL1
VVAVTYSPGPHLERFLASLSLATERPVSVLLADNGSTDGTPQAAVQRYPNVRLLPTGANLGYGTAVNRTIAQLGEMAGDAGEPWVDDWVIVANPDVQWGPGSIDALLDAASRWPRAGALGPLIRDPDGSVYPSARQMPSLIRGGMHAVLGPFWPRNPWTTAYRQERLEPSERPVGWLSGSCLLVRRSAFGQVGGFDERYFMYMEDVDLGDRLGKAGWLSVYVPSAEVLHHKAHSTGRDPASHLAAHHKSTYIFLADRHSGWWRAPLRWTLRGSLALRSHLMVRSSLRRSRRRKLKLVEGRH
Bibliography
- Rubirés X et al. [1997]. A gene (wbbL) from Serratia marcescens N28b (O4) complements the rfb-50 mutation of Escherichia coli K-12 derivatives. Homolog Mutant Function
- Valvano MA, Messner P and Kosma P [2002]. Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides. Review Function
- Ma Y et al. [2002]. Formation of dTDP-rhamnose is essential for growth of mycobacteria. Homolog Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
