Gene Rv3338
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3338, (MTV016.38), len: 214 aa. Hypothetical protein, equivalent to C-termini of Q49926|ML0685 TPEA (putative hydrolase) from Mycobacterium leprae (303 aa), FASTA scores: opt: 984, E(): 2.6e-56, (65.4% identity in 214 aa overlap); and O32873|MLCB1779.02 hypothetical 31.8 KDA protein (similar to alpha/beta hydrolase fold) from Mycobacterium leprae (292 aa), FASTA scores: opt: 984, E(): 2.5e-56, (65.4% identity in 214 aa overlap). Also similar to C-termini of several hypothetical proteins (generally hydrolases) e.g. Q9K3H6|2SCG18.11 putative hydrolase from Streptomyces coelicolor (316 aa), FASTA scores: opt: 213, E(): 1.4e-06, (29.75% identity in 185 aa overlap). Homology suggests that this ORF should be in frame with the previous ORF MTV016.37 but no sequence error could be found. |
| Functional category | Conserved hypotheticals |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3723904 | 3724548 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3338|Rv3338
LSSAVLADHVERQLDELGWETSHIVGNSLGGWVAFELERRGRARSVTGIAPAGGWTRWSPVKFEVIAKFIAGAPILAVAHILGQRALRLPFSRLLATLPISATPDGVSERELSGIIDDAAHCPAYFQLLVKALVLPGLQELEHTAVPSHVVLCEQDRVVPPSRFSRHFTDSLPAGHRLTVLDGVGHVPMFEAPGRITELITSFIEECCPHVRAS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
