Gene Mb3370
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | CONSERVED HYPOTHETICAL PROTEIN |
| Comments | Mb3370, -, len: 297 aa. Equivalent to Rv3337 and Rv3338, len: 128 aa and 214 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 81 aa overlap and 100.0% identity in 214 aa overlap). Rv3337: Conserved hypothetical protein, equivalent to N-terminus of Q49926|ML0685 TPEA (PUTATIVE HYDROLASE) from Mycobacterium leprae (303 aa), FASTA scores: *****opt: 362, E(): 3e-17, (74.3% identity in 70 aa overlap). Also weak similarity in N-terminus to BAB49078|MLR1789 PROBABLE EPOXIDE HYDROLASE from Rhizobium loti (Mesorhizobium loti) (300 aa), FASTA scores: opt: 116, E(): 0.74, (38.9% identity in 54 aa overlap). Homology suggests this ORF should be in frame with the following ORF MTV016.38 but no sequence error could be found. Short distance to start of trpS suggests region may not be protein-coding. C-terminus extended since first submission (+47 aa). Rv3338: Hypothetical protein, equivalent to C-termini of Q49926|ML0685 TPEA (PUTATIVE HYDROLASE) from Mycobacterium leprae (303 aa), FASTA scores: opt: 984, E(): 2.6e-56,(65.4% identity in 214 aa overlap); and O32873|MLCB1779.02 HYPOTHETICAL 31.8 KDA PROTEIN (SIMILAR TO ALPHA/BETA HYDROLASE FOLD) from Mycobacterium leprae (292 aa), FASTA scores: opt: 984, E(): 2.5e-56, (65.4% identity in 214 aa overlap). Also similar to C-termini of several hypothetical proteins (generally hydrolases) e.g. Q9K3H6|2SCG18.11 PUTATIVE HYDROLASE from Streptomyces coelicolor (316 aa), FASTA scores: opt: 213, E(): 1.4e-06,(29.75% identity in 185 aa overlap). Homology suggests that this ORF should be in frame with the previous ORF MTV016.37 but no sequence error could be found. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv3337 and Rv3338 exist as 2 genes with an overlap region between them. In Mycobacterium bovis, a single base insertion (*-t) leads to a single product. |
| Functional category | Conserved hypotheticals |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3680905 | 3681798 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3370|Mb3370
MPSPSTTGHHAACGTGGTGFSVGSMRSPIRVGSGEPVLLLHPFLMSQTVWEKVAQQLADTGRFEVFAPTMAGHNGGPASGTRFLSSAVLADHVERQLDELGWETSHIVGNSLGGWVAFELERRGRARSVTGIAPAGGWTRWSPVKFEVIAKFIAGAPILAVAHILGQRALRLPFSRLLATLPISATPDGVSERELSGIIDDAAHCPAYFQLLVKALVLPGLQELEHTAVPSHVVLCEQDRVVPPSRFSRHFTDSLPAGHRLTVLDGVGHVPMFEAPGRITELITSFIEECCPHVRAS
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