Gene Rv3393
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in purine salvage. Catalyzes the hydrolysis of all of the commonly occurring purine and pyrimidine nucleosides into ribose and the associated base, and could have a preference for inosine and uridine as substrates [catalytic activity: a N-D-ribosylpurine + H(2)O = a purine + D- ribose]. |
| Product | Probable nucleoside hydrolase IunH (purine nucleosidase) |
| Comments | Rv3393, (MTV004.51), len: 308 aa. Probable iunH, nucleoside hydrolase, similar to others e.g. Q9RXB2|DR0403 from Deinococcus radiodurans (314 aa), FASTA scores: opt: 497, E(): 6e-24, (34.3% identity in 312 aa overlap); Q27546|IUNH_CRIFA from Crithidia fasciculata (314 aa), FASTA scores: opt: 475, E(): 1.4e-22, (31.45% identity in 318 aa overlap); Q9CK67|IUNH from Pasteurella multocida (310 aa), FASTA scores: opt: 464, E(): 6.9e-22, (30.9% identity in 314 aa overlap); Q9A549|CC2615 from Caulobacter crescentus (323 aa), FASTA scores: opt: 464, E(): 7.2e-22, (37.85% identity in 280 aa overlap); etc. Note that also similar to BAB34113|ECS0690 (alias AAG54985|YBEK) putative tRNA synthetase from Escherichia coli strain O157:H7 (311 aa), FASTA scores: opt: 483, E(): 4.5e-23, (33.0% identity in 315 aa overlap). The active site histidine is conserved. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3808461 | 3809387 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3393|iunH
VSVVFADVDTGIDDALAVIYLLASPDADLVGIASTGGNIAVGQVCANNLSLLELCGAADIPVSKGADEPLGGRWPDHPKFHGPKGIGYAELPASNRRLTDYDATTAWIAAAHSHAGDLIGLVTGPLTNLALALRAEPALPRLLRRLVIMGGMFDGQPITEWNIRVDPEAASEVFTAWAGQRQLPIVCGLDLTRRVAMTPDILARLASVCGSSPVMRVIEDALRFYFESHEARGHGYLAYMHDPLAAAVAMDPELLTTRTATVDVDPTGATVTDWSGKRNPNARIGMSVDPAVFFDRFVERIGRFARRT
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
