Gene Rv3400
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Probable hydrolase |
| Comments | Rv3400, (MTCY78.28c), len: 262 aa. Probable hydrolase, strongly equivalent to Q49741|YY00_MYCLE|ML0393|B1620_F3_119 hypothetical 28.6 KDA protein from Mycobacterium leprae (261 aa), FASTA scores: opt: 1293, E(): 2.2e-71, (74.45% identity in 262 aa overlap). Similar to several various proteins (notably hydrolases) e.g. Q9L2I7|SCF42.32 putative hydrolase from Streptomyces coelicolor (246 aa), FASTA scores: opt: 888, E(): 7.7e-47, (56.35% identity in 245 aa overlap); Q9EX06|2SCG38.13 putative hydrolase from Streptomyces coelicolor (238 aa), FASTA scores: opt: 195, E(): 8.1e-05, (29.5% identity in 234 aa overlap); Q9I5X4|PA0562 probable hydrolase from Pseudomonas aeruginosa (224 aa), FASTA scores: opt: 190, E(): 0.00015, (27.8% identity in 248 aa overlap); O06995|PGMB_BACSU|YVDM putative beta-phosphoglucomutase from Bacillus subtilis (226 aa), FASTA scores: opt: 190, E(): 0.00016, (33.9% identity in 245 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), FASTA scores: opt: 413, E(): 2e-17, (34.9% identity in 238 aa overlap). Interestingly, note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | The product of this CDS corresponds to spot 3_236 identified in culture supernatant by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany (see citations below). Identified in the culture supernatant of M. tuberculosis H37Rv using mass spectrometry (See Mattow et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3817239 | 3818027 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3400|Rv3400
MANWYRPNYPEVRSRVLGLPEKVRACLFDLDGVLTDTASLHTKAWKAMFDAYLAERAERTGEKFVPFDPAADYHTYVDGKKREDGVRSFLSSRAIEIPDGSPDDPGAAETVYGLGNRKNDMLHKLLRDDGAQVFDGSRRYLEAVTAAGLGVAVVSSSANTRDVLATTGLDRFVQQRVDGVTLREEHIAGKPAPDSFLRAAELLGVTPDAAAVFEDALSGVAAGRAGNFAVVVGINRTGRAAQAAQLRRHGADVVVTDLAELL
Bibliography
- Jungblut PR, Schaible UE, Mollenkopf HJ, Zimny-Arndt U, Raupach B, Mattow J, Halada P, Lamer S, Hagens K and Kaufmann SH [1999]. Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: towards functional genomics of microbial pathogens. Proteomics
- Mollenkopf HJ et al. [1999]. A dynamic two-dimensional polyacrylamide gel electrophoresis database: the mycobacterial proteome via Internet. Proteomics
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Mattow J, Schaible UE, Schmidt F, Hagens K, Siejak F, Brestrich G, Haeselbarth G, Muller EC, Jungblut PR and Kaufmann SH [2003]. Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
