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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionCatalyses the first reaction unique to GMP biosynthesis [catalytic activity: inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH].
ProductProbable inosine-5'-monophosphate dehydrogenase GuaB3 (imp dehydrogenase) (inosinic acid dehydrogenase) (inosinate dehydrogenase) (imp oxidoreductase) (inosine-5'-monophosphate oxidoreductase) (IMPDH) (IMPD)
CommentsRv3410c, (MTCY78.18), len: 375 aa. Probable guaB3, inosine-5'-monophosphate (imp) dehydrogenase, equivalent to Q49721|YY10_MYCLE|ML0388|B1620_C2_193 hypothetical 38.9 KDA protein from Mycobacterium leprae (375 aa), FASTA scores: opt: 2182, E(): 9.5e-122, (90.6% identity in 373 aa overlap). Highly similar to Q9RHY9 GUAB ORF genes for imp dehydrogenase, hypothetical protein from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (376 aa), FASTA scores: opt: 1490, E(): 7.6e-81, (61.0% identity in 382 aa overlap); Q9L0I6|SCD63.03 putative inosine-5'-monophosphate dehydrogenase from Streptomyces coelicolor (374 aa), FASTA scores: opt: 1275, E(): 3.8e-68, (52.95% identity in 372 aa overlap); P73853|GUAB|SLR1722 imp dehydrogenase subunit from Synechocystis sp. strain PCC 6803 (387 aa), FASTA scores: opt: 882, E(): 6.7e-45, (41.3% identity in 373 aa overlap); and similar to other inosine-5'-monophosphate dehydrogenases e.g. P44334|IMDH_HAEIN|GUAB|HI0221 from Haemophilus influenzae (488 aa), FASTA scores: opt: 267, E(): 1.8e-08, (34.25% identity in 216 aa overlap); etc. Also highly similar to the C-terminus of Q50753|GUAA/B homology to Mycobacterium leprae GUAA (fragment) from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 506, E(): 4.6e-23, (85.05% identity in 87 aa overlap). Similar to other eukaryotic and prokaryotic IMPDH and to GMP reductase.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantDisruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3410c|guaB3