Gene Rv3441c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; involved in cellular metabolism. |
| Product | Probable phospho-sugar mutase / MrsA protein homolog |
| Comments | Rv3441c, (MTCY77.13c), len: 448 aa. Probable mrsA, phosphoglucomutase or phosphomannomutase, equivalent to Q49869|URED|B229_C3_234 MRSA protein homolog from Mycobacterium leprae (463 aa), FASTA scores: opt: 2449, E(): 6.3e-135, (87.65% identity in 445 aa overlap); and highly similar (but longer 178 aa) to Q49862|UREC|B229_C2_192 putative urease operon UREC protein from Mycobacterium leprae (288 aa), FASTA scores: opt: 1442, E(): 1.3e-76, (86.5% identity in 267 aa overlap). Highly similar to phospho-sugar mutases e.g. Q53876|SC6G4.14 putative phospho-sugar mutase (similar to phosphomannomutases) from Streptomyces coelicolor (452 aa), FASTA scores: opt: 1710, E(): 5e-92, (60.45% identity in 450 aa overlap); Q9KG46|BH0267 phosphoglucosamine mutase from Bacillus halodurans (447 aa), FASTA scores: opt: 1351, E(): 3.5e-71, (48.4% identity in 444 aa overlap); BAB58323|GLMM phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus Mu50 (451 aa) and Q99QR5|GLMM(FEMD)|SA1965 phosphoglucosamine-mutase from Staphylococcus aureus subsp. aureus N315. (451 aa), FASTA scores: opt: 1315, E(): 4.3e-69, (48.45% identity in 446 aa overlap); P95685|FEMD|GLMM phosphoglucosamine-mutase (451 aa), FASTA scores: opt: 1310, E(): 8.5e-69, (48.2% identity in 446 aa overlap); P95575|MRSA_PSESY MRSA protein homolog from Pseudomonas syringae (pv. syringae) (447 aa), FASTA scores: opt: 1143, E(): 4.2e-59, (42.75% identity in 447 aa overlap); etc. Contains PS00710 Phosphoglucomutase and phosphomannomutase phosphoserine signature. Belongs to the phosphohexose mutases family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3860024 | 3861370 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3441c|mrsA
MGRLFGTDGVRGVANRELTAELALALGAAAARRLSRSGAPGRRVAVLGRDPRASGEMLEAAVIAGLTSEGVDALRVGVLPTPAVAYLTGAYDADFGVMISASHNPMPDNGIKIFGPGGHKLDDDTEDQIEDLVLGVSRGPGLRPAGAGIGRVIDAEDATERYLRHVAKAATARLDDLAVVVDCAHGAASSAAPRAYRAAGARVIAINAEPNGRNINDGCGSTHLDPLRAAVLAHRADLGLAHDGDADRCLAVDANGDLVDGDAIMVVLALAMKEAGELACNTLVATVMSNLGLHLAMRSAGVTVRTTAVGDRYVLEELRAGDYSLGGEQSGHIVMPALGSTGDGIVTGLRLMTRMVQTGSSLSDLASAMRTLPQVLINVEVVDKATAAAAPSVRTAVEQAAAELGDTGRILLRPSGTEPMIRVMVEAADEGVAQRLAATVADAVSTAR
Bibliography
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
