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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionFormation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAS [catalytic activity: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O].
ProductProbable tRNA pseudouridine synthase a TruA (pseudouridylate synthase I) (pseudouridine synthase I) (uracil hydrolyase)
CommentsRv3455c, (MTCY13E12.08c), len: 256 aa. Probable truA, pseudouridine synthase A, equivalent to Q9X796|TRUA_MYCLE|ML1955|MLCB1222.25c tRNA pseudouridine synthase a from Mycobacterium leprae (249 aa), FASTA scores: opt: 1345, E(): 3.2e-80, (77.25% identity in 246 aa overlap). Also highly similar to others e.g. O86776|TRUA_STRCO|SC6G4.09 from Streptomyces coelicolor (284 aa), FASTA scores: opt: 595, E(): 1.7e-31, (49.8% identity in 259 aa overlap); Q9RS37|DR2290 from Deinococcus radiodurans (280 aa), FASTA scores: opt: 383, E(): 1e-17, (41.2% identity in 216 aa overlap); Q9PJT0|TRUA_CHLMU|TC0748 from Chlamydia muridarum (267 aa), FASTA scores: opt: 334, E(): 1.5e-14, (37.65% identity in 231 aa overlap); P07649|TRUA_ECOLI|hist|ASUC|LEUK|B2318 from Escherichia coli strain K12 (270 aa), FASTA scores: opt: 315, E(): 2.5e-13, (33.35% identity in 240 aa overlap); etc. Belongs to the TruA family of pseudouridine synthases.
Functional categoryInformation pathways
ProteomicsIdentified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3455c|truA