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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionCould be involved in valine and isoleucine biosynthesis (at the first step) [catalytic activity: 2-acetolactate + CO(2) = 2 pyruvate].
ProductProbable acetohydroxyacid synthase IlvX (acetolactate synthase)
CommentsRv3509c, (MTV023.16), len: 515 aa. Probable ilvX, acetohydroxyacid synthase, equivalent to Mycobacterium leprae protein described as Acetolactate synthase I, valine sensitive, large subunit Q49865|ILVX|ILVI1|B229_C3_222 (515 aa), FASTA scores: opt: 2762, E(): 8.8e-145, (82.9% identity in 515 aa overlap). Also similar to various enzymes (principally acetohydroxyacid/acetolactate synthases) e.g. Q9AB41|CC0393 thiamine-pyrophosphate-requiring enzyme from Caulobacter crescentus (512 aa), FASTA scores: opt: 1572, E(): 2.8e-79, (50.95% identity in 514 aa overlap); BAB50432|MLL3567 acetolactate synthase I from Rhizobium loti (Mesorhizobium loti) (517 aa), FASTA scores: opt: 1440, E(): 5.2e-72, (47.9% identity in 548 aa overlap); P20906|MDLC_PSEPU benzoylformate decarboxylase from Pseudomonas putida (528 aa), FASTA scores: opt: 356, E(): 2.5e-12, (28.1% identity in 530 aa overlap); Q9L123|SC6D11.33c putative decarboxylase from Streptomyces coelicolor (526 aa), FASTA scores: opt: 325, E(): 1.3e-10, (33.2% identity in 530 aa overlap); Q9RDF9|SCC57A.40c putative acetolactate synthase from Streptomyces coelicolor (564 aa), FASTA scores: opt: 304, E(): 1.9e-09, (28.55% identity in 550 aa overlap); P94783 valine-sensitive acetohydroxy acid synthase from Citrobacter freundii (561 aa), FASTA scores: opt: 278, E(): 5.1e-08, (25.8% identity in 550 aa overlap); Q42767|AHAS acetohydroxyacid synthase from Gossypium hirsutum (Upland cotton) (659 aa), FASTA scores: opt: 278, E(): 5.8e-08, (26.15% identity in 558 aa overlap); etc. Note that other Mycobacterium tuberculosis proteins, e.g. O53250|MTV012.17c|ILVB_MYCTU|Rv3003c|MT3083|MTV012.17c, show better similarity to Acetolactate synthase I. Similar to other enzymes which require TPP. Cofactor: thiamin pyrophosphate (by similarity).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany (See Jungblut et al., 1999). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3509c|ilvX