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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown; probably involved in cellular metabolism.
ProductPossible coenzyme F420-dependent oxidoreductase
CommentsRv3520c, (MTV023.27c), len: 347 aa. Possible coenzyme F420-dependent oxidoreductase, equivalent to Q9CCV8|ML0348 possible coenzyme F420-dependent oxidoreductase from Mycobacterium leprae (350 aa), FASTA scores: opt: 2029, E(): 9.1e-120, (86.85% identity in 342 aa overlap). Similar to many coenzyme F420-dependent enzymes (and other proteins) e.g. Q9AD98|SCI52.11c putative ATP/GTP-binding protein from Streptomyces coelicolor (351 aa), FASTA scores: opt: 859, E(): 1.6e-46, (41.9% identity in 346 aa overlap); Q9X7Y1|SC6A5.35 putative oxidoreductase from Streptomyces coelicolor (341 aa), FASTA scores: opt: 800, E(): 7.9e-43, (38.95% identity in 339 aa overlap); Q9ZA30|GRA-ORF29 putative FMN-dependent monooxygenase from Streptomyces violaceoruber (343 aa), FASTA scores: opt: 354, E(): 6.7e-15, (34.2% identity in 336 aa overlap); Q49598|mer coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase from Methanopyrus kandleri (349 aa), FASTA scores: opt: 283, E(): 1.9e-10, (26.75% identity in 329 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 227, E(): 5.8e-07, (26.35% identity in 334 aa overlap); O27784|MTH1752 coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (321 aa), FASTA scores: opt: 207, E(): 1e-05, (27.4% identity in 336 aa overlap); etc. Also similar to Q11030|YD60_MYCTU|Rv1360|MT1405|MTCY02B10.24 hypothetical 37.3 KDA protein from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 313, E(): 2.5e-12, (28.0% identity in 311 aa overlap).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS39563253957368-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3520c|Rv3520c
VEAGMKLGLQLGYWGAQPPQNHAELVAAAEDAGFDTVFTAEAWGSDAYTPLAWWGSSTQRVRLGTSVIQLSARTPTACAMAALTLDHLSGGRHILGLGVSGPQVVEGWYGQRFPKPLARTREYIDIVRQVWARESPVTSAGPHYRLPLTGEGTTGLGKALKPITHPLRADIPIMLGAEGPKNVALAAEICDGWLPIFYSPRMAGMYNEWLDEGFARPGARRSREDFEICATAQVVITDDRAAAFAGIKPFLALYMGGMGAEETNFHADVYRRMGYTQVVDEVTKLFRSGRKDEAAEIIPDELVDDAVIVGDIDHVRKQMAVWEAAGVTMMVVTAGSAEQVRDLAALV