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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionSupposed involvement in one, or several, catabolic pathways [catalytic activity: 4-hydroxy-2-oxovalerate = pyruvate + acetaldehyde]. Predicted to be involved in lipid catabolism.
ProductProbable 4-hydroxy-2-oxovalerate aldolase (HOA)
CommentsRv3534c, (MTCY03C7.22), len: 346 aa. Probable hsaF, 4-hydroxy-2-oxovalerate aldolase, highly similar to others e.g. P51015|BPHI_PSESP from Pseudomonas sp. strain LB400 (346 aa), FASTA scores: opt: 1150, E(): 2.3e-61, (51.35% identity in 331 aa overlap); Q52040|BPHX3 from Pseudomonas pseudoalcaligenes (346 aa), FASTA scores: opt: 1147, E(): 3.5e-61, (51.35% identity in 331 aa overlap); P51017|NAHM_PSEPU from Pseudomonas putida (346 aa), FASTA scores: opt: 1145, E(): 4.7e-61, (50.9% identity in 330 aa overlap) (see citation below); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 1133, E(): 2.4e-60, (52.0% identity in 327 aa overlap); O24833|ATDG from Acinetobacter sp (340 aa), FASTA scores: opt: 1132, E(): 2.7e-60, (50.45% identity in 331 aa overlap); etc. Note that also highly similar to Q9ZI56|NAHM 2-oxo-4-hydroxypentanoate aldolase from Pseudomonas stutzeri (Pseudomonas perfectomarina) (346 aa) FASTA scores: opt: 1168, E(): 2e-62, (51.05% identity in 331 aa overlap) (see citation below).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS39725523973592-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3534c|hsaF
MTDMWDVRITDTSLRDGSHHKRHQFTKDEVGAIVAALDAAGVPVIEVTHGDGLGGSSFNYGFSKTPEQELIKLAAATAKEARIAFLMLPGVGTKDDIKEARDNGGSICRIATHCTEADVSIQHFGLARELGLETVGFLMMAHTIAPEKLAAQARIMADAGCQCVYVVDSAGALVLDGVADRVSALVAELGEDAQVGFHGHENLGLGVANSVAAVRAGAKQIDGSCRRFGAGAGNAPVEALIGVFDKIGVKTGIDFFDIADAAEDVVRPAMPAECLLDRNALIMGYSGVYSSFLKHAVRQAERYGVPASALLHRAGQRKLIGGQEDQLIDIALEIKRELDSGAAVTH
      
Bibliography