Gene Rv3561
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable fatty-acid-CoA ligase FadD3 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase) |
| Comments | Rv3561, (MTCY06G11.08), len: 507 aa. Probable fadD3, fatty-acid-CoA synthetase, similar to many substrate-CoA symthetases/ligases e.g. Q9KBC2|BH2006 long-chain acyl-CoA synthetase from Bacillus halodurans (513 aa), FASTA scores: opt: 821, E(): 1.6e-43, (32.9% identity in 517 aa overlap); Q9EY88|FCS feruloyl-CoA synthetase from Amycolatopsis sp. HR167 (491 aa) FASTA scores: opt: 767, E(): 3.5e-40, (37.65% identity in 502 aa overlap); Q9ZIP5|MATB malonyl CoA synthetase from Rhizobium leguminosarum (504 aa), FASTA scores: opt: 758, E(): 1.3e-39, (33.7% identity in 472 aa overlap); Q9CD27|FADD2|ML2546 acyl-CoA synthase from Mycobacterium leprae (548 aa), FASTA scores: opt: 700, E(): 5.6e-36, (31.85% identity in 515 aa overlap); P29212|LCFA_ECOLI|FADD|OLDD|B1805 long-chain-fatty-acid--CoA ligase from Escherichia coli strain K12 (561 aa), FASTA scores: opt: 532, E(): 6.3e-28, (30.0% identity in 533 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg O53306|FADD13|Rv3089|MTV013.10 (503 aa), FASTA scores: opt: 819, E(): 2.1e-43, (35.1% identity in 490 aa overlap). Contains PS00455 Putative AMP-binding domain signature. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4001637 | 4003160 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3561|fadD3
LINDLRTVPAALDRLVRQLPDHTALIAEDRRFTSTELRDAVYGAAAALIALGVEPADRVAIWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAAGLFLGADRAAGLDRAALPALRHVVRVPVEADDGTWDEFIATGAGALDAVAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKITSDDRYLCINPFFHNFGYKAGILACLQTGATLIPHVTFDPLHALRAIERHRITVLPGPPTIYQSLLDHPARKDFDLSSLRFAVTGAATVPVVLVERMQSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRIADDGEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGDIGAVDQAGNLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAVIGVPDQRLGEVGRAFVVARPGTGLDEASVIAYTREHLANFKTPRSVRFVDVLPRNAAGKVSKPQLRELG
Bibliography
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
