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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown, but involved in lipid degradation.
ProductProbable acyl-CoA dehydrogenase FadE33
CommentsRv3564, (MTCY06G11.11), len: 318 aa. Probable fadE33, acyl-CoA dehydrogenase, similar to others e.g. Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 373, E(): 1.9e-15, (34.3% identity in 338 aa overlap); Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 277, E(): 1.4e-09, (31.95% identity in 335 aa overlap); Q9X7Y6|SC6A5.40c from Streptomyces coelicolor (395 aa), FASTA scores: opt: 273, E(): 2.5e-09, (30.1% identity in 352 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 478, E(): 7.9e-22, (32.9% identity in 292 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96845|FADE32|Rv3563|MTCY06G11.10 (319 aa), FASTA scores: opt: 478, E(): 7.9e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family.
Functional categoryLipid metabolism
ProteomicsIdentified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS40052474006203+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3564|fadE33
MTPPEERQMLRETVASLVAKHAGPAAVRAAMASDRGYDESLWRLLCEQVGAAALVIPEELGGAGGELADAAIVVQELGRALVPSPLLGTTLAELALLAAAKPDAQALTELAQGSAIGALVLDPDYVVNGDIADIVVAATSGQLTRWTRFSAQPVATMDPTRRLARLQSEETEPLCPDPGIADTAAILLAAEQIGAAERCLQLTVEYAKSRVQFGRPIGSFQALKHRMADLYVTIAAARAVVADACHAPTPTNAATARLAASEALSTAAAEGIQLHGGIAITWEHDMHLYFKRAHGSAQLLESPREVLRRLESEVWESP