Gene Rv3645
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown. Inhibited by polyphosphates. |
| Product | Probable conserved transmembrane protein |
| Comments | Rv3645, (MTCY15C10.07c), len: 549 aa. Probable conserved transmembrane protein, equivalent, but longer 20 aa, to O69547|ML0201|MLCB2548.30 putative membrane protein from Mycobacterium leprae (530 aa), FASTA scores: opt: 2958, E(): 1.5e-168, (85.5% identity in 530 aa overlap). Also closely related to several other hypothetical M. tuberculosis proteins, e.g. Q10631|YD18_MYCTU|Rv1318c|MT1359|MTCY130.03c (541 aa) FASTA scores: opt: 1105, E(): 2.7e-58, (39.35% identity in 506 aa overlap); Q10633|YD20_MYCTU|Rv1320c|MT1362|MTCY130.05c (567 aa) FASTA scores: opt: 1031, E(): 7.1e-54, (38.1% identity in 509 aa overlap); Q10632|YD19_MYCTU|Rv1319c|MTCY130.04c (535 aa), FASTA scores: opt: 1016, E(): 5.3e-53, (37.1% identity in 531 aa overlap); etc. Also similar at C-terminal end to many adenylate cyclases e.g. O83498|TP0485 from Treponema pallidum (614 aa) FASTA scores: opt: 365, E(): 3.2e-14, (31.55% identity in 317 aa overlap); P94180|CYAA from Anabaena sp. strain PCC 7120 (735 aa), FASTA scores: opt: 364, E(): 4.2e-14, (32.75% identity in 229 aa overlap); etc. |
| Functional category | Cell wall and cell processes |
| Mutant | Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4082807 | 4084456 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3645|Rv3645
MDAEAFVGFRQVPAARYGGLMATTAALPRRIHAFVRWVVRTPWPLFSLSMLQSDIIGALFVLGFLRYGLPPQDNIQLQDLPPVNLLIFVSTVIILFLAGAVVNLKLLMPVFRWQRRDNLLTEPDPAATELARSRALRMPLYRTLISLAVWATGGGVFILASWSVAKHAAPVVAVATALGATATAIIGYLQSERVLRPVAVAALRSGVPENVNAPGVILRLMLAWIPSTGVPLLAIVLAVAADKIALLHATPEALFNPILMMALAALGIGSVSTLLVAMSIADPLRQLRWALSEVQRGNYNAHMQIYDASELGLLQAGFNDMVRELSERQRLRDLFGRYVGEDVARRALERGTELGGQERDVAVLFVDLVGSTQLAATRPPAEVVQLLNEFFRVVVETVARHGGFVNKFQGDAALAIFGAPIEHPDGAGAALSAARELHDELIPVLGSAEFGIGVSAGRAIAGHIGAQARFEYTVIGDPVNEAARLTELAKLEDGHVLASAIAVSGALDAEALCWDVGEVVELRGRAAPTQLARPMNLAAPEEVSSEVRG
Bibliography
- Guo YL et al. [2009]. Polyphosphates from Mycobacterium bovis--potent inhibitors of class III adenylate cyclases. Biochemistry
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
