Gene Rv3649
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly has helicase activity. |
| Product | Probable helicase |
| Comments | Rv3649, (MTCY15C10.03c), len: 771 aa. Probable helicase, similar to many (known or hypothetical) ATP-dependent helicases e.g. Q9X915|SCH5.13 putative helicase from Streptomyces coelicolor (815 aa) FASTA scores: opt: 2550, E(): 9.6e-139, (52.45% identity in 774 aa overlap); Q05549|YDR291W|D9819.1 protein similar to several DNA helicases from Saccharomyces cerevisiae (Baker's yeast) (1077 aa), FASTA scores: opt: 1161, E(): 5.9e-59, (31.05% identity in 780 aa overlap); P50830|YPRA_BACSU hypothetical helicase from Bacillus subtilis (749 aa), FASTA scores: opt: 1154, E(): 1.1e-58, (34.05% identity in 734 aa overlap); Q9KC10|BH1764 ATP-dependent RNA helicase from Bacillus halodurans (764 aa), FASTA scores: opt: 1122, E(): 8e-57, (32.3% identity in 759 aa overlap); etc. Seems similar to dead/DEAH box helicase family, and to helicase C-terminal domain. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4088781 | 4091096 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3649|Rv3649
MASFGSHLLAAAVAGTPPGERPLRHVAELPPQAGRPRGWPEWAEPDVVDAFADRGISSPWSHQAEAAELAYAGRHVVIGTGPASGKSLAYQLLVLNALATDSRARALYLSPTKALGHDQLRAAHALAAAVPRLADVAPTAYDGDSPDEVRRFARERSRWLFSNPEMTHLSVLRNHARWAVLLRNLRFVIVDECHYYRGVFGSNVAMVLRRLLRLCARYSAHPTVIFASATTASPGATAADLIGQPVVEVTEDGSPRGARTVALWEPALRSDVIGEHGAPVRRSAGAEAARVMADLIVEGAQTLTFVRSRRAAELTALGARARLVDIAPELSDTVASYRAGYLAEDRSALHQALAEGQLRGLATTNALELGVDIAGLDAVVLAGFPGTVASFWQQAGRSGRRGQGALVVLIARDDPLDTYLVHHPAALLDKPVERVVIDPVNPHLLGPQLLCAATELPLDDAEVRSWGAVEVAESLVDDGLLRRRNGRYFPAPGVKPHAAVDVRGAIGGQIVIVEAGTGRLLGSVGVGQAPAAAHPGAVYLHQGETYVVDSLDFQDGIAFVHAEDPGYATFAREVTDIAVTGTGERLVFGPVALGLVPVTVTNHVVGYLRRQLSGEVLDFVELDMPEHTLPTTAVMYTITSDALVRSGIEATRIPGSLHAAEHAAIGLLPLVASCDRGDIGGMSTATGPEGLPSVFVYDGYPGGAGFAERGFRRARTWLGATAEAIEACECPSGCPSCVQSPKCGNGNDPLDKAGAVRVLRLVLAELSEESP
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
