Gene Rv3696c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Acts in rate-limiting step in glycerol utilization. Key enzyme in the regulation of glycerol uptake and metabolism [catalytic activity: ATP + glycerol = ADP + glycerol 3-phosphate]. |
| Product | Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) (glycerokinase) (GK) |
| Comments | Rv3696c, (MTV025.044c), len: 517 aa. Probable glpK, glycerol kinase, equivalent to Q9CB81|GLPK_MYCLE|ML2314 glycerol kinase from Mycobacterium leprae (508 aa), FASTA scores: opt: 3120, E(): 4.7e-189, (91.35% identity in 508 aa overlap). Also highly similar to others e.g. Q9RJM2|GLPK from Streptomyces coelicolor (507 aa), FASTA scores: opt: 2606, E(): 1.1e-156, (75.35% identity in 503 aa overlap); Q9ADA7|GLPK from Streptomyces coelicolor (512 aa) FASTA scores: opt: 2002, E(): 1.3e-118, (59.05% identity in 503 aa overlap); Q9X1E4|GLK2_THEMA|TM1430 from Thermotoga maritima (496 aa), FASTA scores: opt: 1838, E(): 2.7e-108, (54.8% identity in 498 aa overlap); P08859|GLPK_ECOLI|B3926 from Escherichia coli strain K12 (501 aa), FASTA scores: opt: 1740, E(): 4.1e-102, (52.3% identity in 499 aa overlap); etc. Contains PS00933 FGGY family of carbohydrate kinases signature 1, PS00070 Aldehyde dehydrogenases cysteine active site, PS00445 FGGY family of carbohydrate kinases signature 2. Belongs to the fucokinase / gluconokinase / glycerokinase / xylulokinase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Slow growth mutant by Himar1-based transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4138202 | 4139755 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3696c|glpK
VSDAILGEQLAESSDFIAAIDQGTTSTRCMIFDHHGAEVARHQLEHEQILPRAGWVEHNPVEIWERTASVLISVLNATNLSPKDIAALGITNQRETTLVWNRHTGRPYYNAIVWQDTRTDRIASALDRDGRGNLIRRKAGLPPATYFSGGKLQWILENVDGVRAAAENGDALFGTPDTWVLWNLTGGPRGGVHVTDVTNASRTMLMDLETLDWDDELLSLFSIPRAMLPEIASSAPSEPYGVTLATGPVGGEVPITGVLGDQHAAMVGQVCLAPGEAKNTYGTGNFLLLNTGETIVRSNNGLLTTVCYQFGNAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQSEALARQVPDNGGMYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLEAICYQSRDVVDAMEADSGVRLQVLKVDGGITGNDLCMQIQADVLGVDVVRPVVAETTALGVAYAAGLAVGFWAAPSDLRANWREDKRWTPTWDDDERAAGYAGWRKAVQRTLDWVDVS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Titgemeyer F et al. [2007]. A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis. Homology
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
